PubMed Cain CW et al., Genetics, 2012 Feb;190(2):511-21Cain CW et al., Genetics, 2012 Feb;190(2):511-21
SIGMA2000
Direct
EMSA
Yhr177w amplified from genomic DNA (SIGMA2000 backgorund) and cloned in a pET28b derivative plasmid. EMSA was performed using the 50-bp 1175-1225 DNA fragment from FLO11 promoter or the mutated version and Yhr177w ( protein concentration range from 0.5 to 4nM)