Yeastract IST INESC-ID IBB-BSRG
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Transcription Factor Binding Site Reference(s)

The Yeastract team, is actively curating each transcriptor factor binding site evidence code and environmental condition.
Transcription
Factor
Binding Site Source References Evidence
Code
Environmental
Condition
Crz1p CAGCCAC BSRG curated PubMed external link Serra-Cardona A et al., Mol Cell Biol, 2014;34(24):4420-35Serra-Cardona A et al., Mol Cell Biol, 2014;34(24):4420-35 Direct: ChIP Chromatin-immunoprecipitated material from cells exposed to pH 8.0 and subjected to PCR amplification for the 143-nt region encompassing the CDRE2 consensus. ChIP samples were subjected to massive sequencing. Mapped reads were quantified, normalized, and represented by using SeqMonk software
Indirect: lacZ reporter assays: wt pPHO89-LacZ vs wt pPHO89CDRE2-LacZ (mutated CAGTAAC) Wild-type strain DBY746 was transformed with plasmid pPHO89-LacZ or pPHO89CDRE2-LacZ (mutated CAGTAAC), and cultures were subjected to pH 8.0.

Consensus sequences are
represented according to
the following IUPAC
frequency table:

IUPAC frequency table
A C G T
A 1 0 0 0
C 0 1 0 0
G 0 0 1 0
T 0 0 0 1
W1/2 0 0 1/2
S 0 1/21/2 0
R1/2 0 1/2 0
Y 0 1/2 0 1/2
M1/21/2 0 0
K 0 0 1/21/2
B 0 1/31/31/3
D1/3 0 1/31/3
H1/31/3 0 1/3
V1/31/31/3 0
N1/41/41/41/4
x1/41/41/41/4
a1/21/61/61/6
c1/61/21/61/6
g1/61/61/21/6
t1/61/61/61/2
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