Yeastract IST INESC-ID IBB-BSRG
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Transcription Factor Binding Site Reference(s)

The Yeastract team, is actively curating each transcriptor factor binding site evidence code and environmental condition.
Transcription
Factor
Binding Site Source References Evidence
Code
Environmental
Condition
Hot1p GGGACAAA BSRG curated PubMed external link Gomar-Alba M et al., Biochim Biophys Acta, 2015;1849(12):1385-97Gomar-Alba M et al., Biochim Biophys Acta, 2015;1849(12):1385-97 Direct: EMSA (GST-tagged Hot1 DNA-binding domain) Kept in exponential phase for 16h
Indirect: One-hybrid spot assay (Hot1 DBD fused to GAL4AD) kept in exponential phase for 16h; SC + his, SC-his and SC-his containing 1 mM 3-amino-1,2,4-triazole (3AT) plates

Consensus sequences are
represented according to
the following IUPAC
frequency table:

IUPAC frequency table
A C G T
A 1 0 0 0
C 0 1 0 0
G 0 0 1 0
T 0 0 0 1
W1/2 0 0 1/2
S 0 1/21/2 0
R1/2 0 1/2 0
Y 0 1/2 0 1/2
M1/21/2 0 0
K 0 0 1/21/2
B 0 1/31/31/3
D1/3 0 1/31/3
H1/31/3 0 1/3
V1/31/31/3 0
N1/41/41/41/4
x1/41/41/41/4
a1/21/61/61/6
c1/61/21/61/6
g1/61/61/21/6
t1/61/61/61/2
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