Yeastract IST INESC-ID IBB-BSRG
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Transcription Factor Binding Site Reference(s)

The Yeastract team, is actively curating each transcriptor factor binding site evidence code and environmental condition.
Transcription
Factor
Binding Site Source References Evidence
Code
Environmental
Condition
Nrg1p CCCCT BSRG curated PubMed external link Park SH et al., Mol Cell Biol, 1999;19(3):2044-50Park SH et al., Mol Cell Biol, 1999;19(3):2044-50
PubMed external link Zhao Y et al., J Mol Cell Biol, 2013;5(5):336-44Zhao Y et al., J Mol Cell Biol, 2013;5(5):336-44 Direct: Electrophoretic mobility shift assay (EMSA): GST alone vs GST-Nrg1 GST-Nrg1 proteins were purified as described (Platara et al., 2006). Double-stranded N1 and N2 probes were made by annealing two pairs of oligonucleotides, PMR 1-1F/PMR 1-1R and PMR 1-2F/PMR 1-2R, respectively, and labeled at the 3 end with the DIG Gel Shift Kit (Roche) according to the manufacturers recommendations.

Consensus sequences are
represented according to
the following IUPAC
frequency table:

IUPAC frequency table
A C G T
A 1 0 0 0
C 0 1 0 0
G 0 0 1 0
T 0 0 0 1
W1/2 0 0 1/2
S 0 1/21/2 0
R1/2 0 1/2 0
Y 0 1/2 0 1/2
M1/21/2 0 0
K 0 0 1/21/2
B 0 1/31/31/3
D1/3 0 1/31/3
H1/31/3 0 1/3
V1/31/31/3 0
N1/41/41/41/4
x1/41/41/41/4
a1/21/61/61/6
c1/61/21/61/6
g1/61/61/21/6
t1/61/61/61/2
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