S.cerevisiae - Yeastract

Regulation Reference(s)

The Yeastract team, is actively curating each regulation evidence code, association type and environmental condition.

Transcription Factor	Target ORF/Genes	References	Evidence Code	Evidence Experiment	Association Type	Strain	Environmental Condition
Gal4p	YBR020W	PubMed Giniger E et al., Cell, 1985 Apr;40(4):767-74	N/A		N/A	S288c	N/A
		PubMed Hashimoto H et al., Mol Gen Genet, 1983;191(1):31-8	N/A		N/A	S288c	N/A
		PubMed West RW Jr et al., Mol Cell Biol, 1984 Nov;4(11):2467-78	Indirect	lacZ - wt vs TF mutant	Positive	S288c	Galactose
		PubMed Vashee S et al., J Biol Chem, 1993 Nov 25;268(33):24699-706	N/A		N/A	S288c	N/A
		PubMed Lee TI et al., Science, 2002 Oct 25;298(5594):799-804	Direct	Chip-on-chip	N/A	S288c	YPD medium; mid-log phase
		PubMed Harbison CT et al., Nature, 2004 Sep 2;431(7004):99-104	Direct	Chip-on-chip	N/A	S288c	YPD medium; mid-log phase
		PubMed Workman CT et al., Science, 2006 May 19;312(5776):1054-9	Direct	Chip-on-chip	N/A	S288c	N/A
		PubMed Kundu S et al., Genes Dev, 2007 Apr 15;21(8):997-1004	Direct	ChIP	N/A	S288c	Galactose- Cells were grown in raffinose media until mid-log phase, and GAL1 was induced for 1 h by adding galactose
		PubMed Archer CT et al., J Biol Chem, 2008 May 2;283(18):12614-23	Direct	EMSA	N/A	S288c	in vitro
		PubMed Prasad V et al., BMC Syst Biol, 2008;():	N/A		N/A	S288c	N/A
		PubMed El Kaderi B et al., J Biol Chem, 2009 Sep 11;284(37):25015-25	Direct	ChIP	N/A	S288c	N/A
		PubMed Collins GA et al., Nature, 2009;():Collins GA et al., Nature, 2009;():	Direct	ChIP	N/A	S288c	Galactose + ?-estradiol/4hydroxytamoxifen
		PubMed Archer CT et al., Nucleic Acids Res, 2010;38(3):789-96	Direct	RT-PCR - wt vs mutant promoter sequence	Positive	S288c	Galactose
		PubMed Li Y et al., Curr Microbiol, 2010 Sep;61(3):210-6	Indirect	lacZ - wt vs TF mutant	Positive	Y187	DELTAdsg1 and DELTAdsg1 cells simultaneously expressing Gal80p and Gal4 T859I or L868P mutant were induced with 2% galactose for 1h.
			Indirect	lacZ - wt vs TF mutant	Positive	JN3	DELTAdsg1 cells expressing wt Gal4p vs various Gal4p derivatives lacking different lengths of the middle region (DELTA683 DELTA600 DELTA500) were induced with 2% galactose for 1 h
			Indirect	lacZ - wt vs TF mutant	Positive	Y187	wt cells co-transformed with pEGGal80 and plasmid expressing wt Gal4p vs Gal4p derivatives K25R together with pSH18-34. Cells were induced with 2% galactose
			Indirect	lacZ - wt vs TF mutant	Negative	Y187	wt cells co-transformed with pEGGal80 and plasmid expressing wt Gal4p vs Gal4p derivatives lacking different lengths of the middle region DELTA683 DELTA600 DELTA500, K23R) together with pSH18-34. Cells were induced with 2% galactose
			Indirect	lacZ - wt vs TF mutant	Positive	JN1	wt cells overexpressing the GAL4 gene were grown in SC medium containing either 5% glycerol and 2% lactate or 2% galactose, at 30.C
			Indirect	lacZ - wt vs TF mutant	Negative	JN1 and JN3	wt vs DELTAdsg1 cells overexpressing the GAL4 gene were grown in SC medium containing either 5% glycerol and 2% lactate or 2% galactose, at 30.C
			Indirect	lacZ - wt vs TF mutant	Negative	JN1 and JN3	wt vs DELTAdsg1DELTAdnm1 cells overexpressing the GAL4 gene were grown in SC medium containing either 5% glycerol and 2% lactate or 2% galactose, at 30.C
		PubMed Dutoit R et al., Nucleic Acids Res, 2010 Oct;38(19):e183	Direct	RT-PCR - wt vs mutant promoter sequence	Positive	S288c	Minimal Media + Galactose
		PubMed Knutson BA et al., Mol Cell Biol, 2011 Feb;31(4):818-31	Direct	ChIP	N/A	S288c	Galactose
		PubMed Campbell RN et al., Yeast, 2011 Aug;28(8):569-78	Indirect	lacZ - wt vs TF mutant	Positive	S288c	raffinose
		PubMed Malik S et al., Nucleic Acids Res, 2012 Apr;40(8):3348-63	Direct	ChIP	N/A	S288c	Galactose
			Direct	ChIP	N/A	S288c	raffinose
		PubMed Abramczyk D et al., Eukaryot Cell, 2012 Mar;11(3):334-42	Direct	ChIP	N/A	S288c	Galactose
		PubMed Shukla A et al., Biochemistry, 2012;():Shukla A et al., Biochemistry, 2012;():	Direct	ChIP	N/A	S288c	Galactose
		PubMed Lin L et al., Proc Natl Acad Sci U S A, 2012 Feb 7;109(6):1997-2002	Direct	ChIP	N/A	S288c	Galactose
			Direct	ChIP	N/A	S288c	raffinose
		PubMed Ryo S et al., Anal Biochem, 2012 May 1;424(1):27-31	Indirect	GFP - wt vs TF overexpression	Positive	S288c	YPGal medium; mid-log phase
		PubMed Dugan A et al., J Am Chem Soc, 2016 Sep 28;138(38):12629-35	Direct	ChIP	N/A	S288c	Grown in 2% glucose to mid-log phase and then induced for 3h in 2% galactose
		PubMed Rossi MJ et al., Nature, 2021 Apr;592(7853):309-314	Direct	ChIP-exo	N/A	BY4741	YPD medium, 25.C