PubMed
Muñoz SA et al., Microbiology (Reading), 2020 Jan;166(1):85-92Muñoz SA et al., Microbiology (Reading), 2020 Jan;166(1):85-92
Direct
ChIP
Positive
26790a
minimal medium containing 0.17% Difco yeast nitrogen base (yeast nitrogen base without amino acids and ammonium sulfate) with 2% glucose as a carbon source and 10mM proline as a nitrogen source + 1.3mM leucine for 1h
Direct
ChIP
N/A
SBCY13
Cells grown on minimal medium with 2% glucose and 10mM proline up to an OD600nm of 0.5-0.9 were harvested and transferred to fresh medium with 1.3 mM leucine for 1 hour.
Indirect
lacZ - wt vs TF mutant
Positive
26790a
minimal medium containing 0.17% Difco yeast nitrogen base (yeast nitrogen base without amino acids and ammonium sulfate) with 2% glucose as a carbon source and 10mM proline as a nitrogen source
Indirect
lacZ - wt vs TF mutant
Positive
26790a
minimal medium containing 0.17% Difco yeast nitrogen base (yeast nitrogen base without amino acids and ammonium sulfate) with 2% glucose as a carbon source and 10mM proline as a nitrogen source + 1.3mM leucine for 1h
Indirect
lacZ - wt vs TF mutant
Positive
26790a
Cells grown on minimal medium with 2% glucose and 10mM ammonium sulfate up to an OD600nm of 0.5-0.9 were harvested and transferred to fresh medium with the same composition for 1 hour.
Indirect
lacZ - wt vs TF mutant
Positive
26790a
Cells grown on minimal medium with 2% glucose and 10mM proline up to an OD600nm of 0.5-0.9 were harvested and transferred to fresh medium with 1.3 mM leucine for 1 hour.
Indirect
lacZ - wt vs TF mutant
Positive
26790a
Cells grown on minimal medium with 2% glucose and 10mM proline up to an OD600nm of 0.5-0.9 were harvested and transferred to fresh medium with the same composition for 1 hour.
Indirect
LacZ - WT vs. TF double mutant
Positive
26790a
Cells grown on minimal medium with 2% glucose and 10mM ammonium sulfate up to an OD600nm of 0.5-0.9 were harvested and transferred to fresh medium with 1.3 mM leucine for 1 hour.