S.cerevisiae - Yeastract

Regulation Reference(s)

The Yeastract team, is actively curating each regulation evidence code, association type and environmental condition.

Transcription Factor	Target ORF/Genes	References	Evidence Code	Evidence Experiment	Association Type	Strain	Environmental Condition
Gln3p	YDL210W	PubMed Talibi D et al., Nucleic Acids Res, 1995 Feb 25;23(4):550-7	Indirect	lacZ - wt vs TF mutant	Positive	S288c	minimal proline medium (0,1% proline) + 0,1% GABA
		PubMed Coffman JA et al., Mol Cell Biol, 1996 Mar;16(3):847-58	Indirect	Northern blot - wt vs TF mutant	Positive	TCY1	Wickerham's minimal medium + proline
		PubMed Cunningham TS et al., J Bacteriol, 1996 Jun;178(12):3470-9	Direct	EMSA	N/A	S288c	in vitro
		PubMed Garcia SC et al., FEMS Microbiol Lett, 2000 Mar 15;184(2):219-24	Indirect	lacZ - wt vs TF mutant	Positive	S288c	Minimal proline medium 2 (MPM2 -in 1 l it contained 0.7 g MgSO4W7H2O, 1 g KH2PO4, 0.4 g CaCl2.2H20, 0.5 g NaCl, 1 g K2SO4, 10.5 g citric acidWH2O, 10 ml vitamin solution (250 mg D-biotin, 100 mg thiamineWHCl, 1 g myo-inositol, 200 mg calcium pantothenate and 100 mg pyridoxineWHCl per liter) and 1 ml of trace metal solution (10 mg H3BO3, 1 mg CuSO4W5H2O, 2 mg KI, 4 mg Na2MoO4W2H2O, 14 mg ZnSO4W7H2O
		PubMed Shamji AF et al., Curr Biol, 2000 Dec 14-28;10(24):1574-81	Indirect	Microarray analysis - wt vs TF mutant	Positive	S288c	30 min 50 nM treatment with rapamycin in YPD
		PubMed Staschke KA et al., J Biol Chem, 2010 May 28;285(22):16893-911	Indirect	Microarray analysis - wt vs TF mutant	Positive	EG328-1A	RNA prepared from wt and DELTAgcn2DELTAgln3 cells treated for 1 h with 200 nM Rapamycin
			Indirect	Microarray analysis - wt vs TF mutant	Positive	EG328-1A	RNA prepared from wt and DELTAgcn4DELTAgln3 cells treated for 1 h with 200 nM Rapamycin
			Indirect	Microarray analysis - wt vs TF mutant	Positive	EG328-1A	RNA prepared from wt and DELTAgln3 cells treated for 1 h with 200 nM Rapamycin
		PubMed Cardillo SB et al., Microbiology, 2012;():	Direct	ChIP	N/A	S288c	Minimal Media + GABA
		PubMed Levi CE et al., Biochem Biophys Res Commun, 2012 May 11;421(3):572-7	Direct	ChIP	N/A	S288c	Exponential phase cells cultivated in minimal growth medium containing acetate and GABA
		PubMed Palavecino-Ruiz M et al., Microbiology, 2017 Nov;163(11):1692-1701	Indirect	lacZ - wt vs TF mutant	Negative	23344c	wt and DELTAgln3DELTAgat1 cells carrying the Yep-357-UGA4-lacZ plasmid were grown on 10mM proline
			Indirect	lacZ - wt vs TF mutant	Negative	30505b	DELTAgln3 cells carrying the Yep-357-UGA4-lacZ plasmid were grown on 10mM proline vs incubated with 0.1mM É£-aminobutyric acid (GABA) for 60 min
			Indirect	lacZ - wt vs TF mutant	Positive	MPY15	wt and DELTAgln3DELTAgat1DELTAleu3 cells carrying the Yep-357-UGA4-lacZ plasmid were grown on 10mM proline and incubated with 0.1mM GABA for 60 min