S.cerevisiae - Yeastract

Regulation Reference(s)

The Yeastract team, is actively curating each regulation evidence code, association type and environmental condition.

Transcription Factor	Target ORF/Genes	References	Evidence Code	Evidence Experiment	Association Type	Strain	Environmental Condition
Uga3p	YDL210W	PubMed Talibi D et al., Nucleic Acids Res, 1995 Feb 25;23(4):550-7	Indirect	lacZ - wt vs TF mutant	Positive	S288c	minimal proline medium (0,1% proline) + 0,1% GABA
		PubMed AndrÃ© B et al., Mol Gen Genet, 1993;():	Indirect	Northern blot - wt vs TF mutant	Positive	S288c	presence of GABA
		PubMed Garcia SC et al., FEMS Microbiol Lett, 2000 Mar 15;184(2):219-24	Indirect	lacZ - wt vs TF mutant	Positive	S288c	Minimal proline medium 2 (MPM2 -in 1 l it contained 0.7 g MgSO4W7H2O, 1 g KH2PO4, 0.4 g CaCl2.2H20, 0.5 g NaCl, 1 g K2SO4, 10.5 g citric acidWH2O, 10 ml vitamin solution (250 mg D-biotin, 100 mg thiamineWHCl, 1 g myo-inositol, 200 mg calcium pantothenate and 100 mg pyridoxineWHCl per liter) and 1 ml of trace metal solution (10 mg H3BO3, 1 mg CuSO4W5H2O, 2 mg KI, 4 mg Na2MoO4W2H2O, 14 mg ZnSO4W7H2O
		PubMed Idicula AM et al., J Biol Chem, 2002 Nov 29;277(48):45977-83	Indirect	lacZ - wt vs TF mutant	Positive	S288c	gamma-aminobutyric acid presence
		PubMed Godard P et al., Mol Cell Biol, 2007 Apr;27(8):3065-86	Direct	RT-PCR - wt vs mutant promoter sequence	Positive	S288c	Urea as nitrogen source + 1h 5mM GABA
		PubMed Cardillo SB et al., Eukaryot Cell, 2010 Aug;9(8):1262-71	Direct	ChIP	N/A	S288c	extracellular leucine and GABA
		PubMed Levi CE et al., Biochem Biophys Res Commun, 2012 May 11;421(3):572-7	Direct	ChIP	N/A	S288c	Exponential phase cells cultivated in minimal growth medium containing glucose and GABA
		PubMed Palavecino-Ruiz M et al., Microbiology, 2017 Nov;163(11):1692-1701	Indirect	RT-PCR - wt vs TF mutant	N/A	23344c	wt, DELTAgln3 and DELTAdal80 cells expressing HA-Uga3 were grown on proline without GABA vs incubated with 0.1 mM GABA for 30 min. ChIPs assays were carried out using antibodies against the HA epitope.