S.cerevisiae - Yeastract

Regulation Reference(s)

The Yeastract team, is actively curating each regulation evidence code, association type and environmental condition.

Transcription Factor	Target ORF/Genes	References	Evidence Code	Evidence Experiment	Association Type	Strain	Environmental Condition
Rim101p	YDR040C	PubMed Serrano R et al., Mol Microbiol, 2002 Dec;46(5):1319-33	Indirect	lacZ - wt vs TF mutant	Positive	S288c	Alkaline pH stress; Cells cultivated in SC medium at pH 8
		PubMed Lamb TM et al., Mol Cell Biol, 2003 Jan;23(2):677-86	Indirect	lacZ - wt vs TF mutant	Positive	SK1	Acidic pH; cells expressing Ena1-lacZ grown to exponential phase in YPD medium at pH 4
		PubMed Lamb TM et al., Mol Cell Biol, 2003 Jan;23(2):677-86	Indirect	lacZ - wt vs TF mutant	Positive	SK1	Alkaline pH; cells expressing Ena1-lacZ grown to exponential phase in YPD medium at pH 8
		PubMed Platara M et al., J Biol Chem, 2006 Dec 1;281(48):36632-42	Indirect	lacZ - wt vs TF mutant	Positive	DBY746	Cells grown in YPD until A660 of 0.5 to 0.7, alkaline stress was provoked by resuspending cells in YPD containing 50 nM TAPS adjusted to pH 8.0 by adding KOH
			Indirect	lacZ - wt vs TF mutant	Positive	DBY746	Cells grown in YPD until A660 of 0.5 to 0.7, resuspended in YPD medium adjusted to pH 6.3
		PubMed Yazawa H et al., Yeast, 2009 Mar;26(3):167-84	Indirect	RT-PCR - wt vs TF mutant	Positive	YHU3201	Alkaline stress - 240 mM K2HPO4
		PubMed MarquÃ©s MC et al., FEMS Yeast Res, 2015 Jun;15(4):fov017	Indirect	Western blot - wt vs TF mutant	Positive	S288c	Strains treated with 0.5 M NaCl for 20 min harbouring a genomic integration of the GFP coding sequence at the ENA1 C-terminus.
		PubMed Anton C et al., Mol Biol Cell, 2017 Dec 1;28(25):3672-3685	Indirect	Western blot - wt vs TF mutant	Positive	CRM67 (W303, mat a, (leu2-3,112 trp1-1 can1-100 ura3-1 ade2-1 his3-11,15))	Levels of Ena1-GFP from its endogenous locus after Indirect of the constitutively processed Rim101C [Lamb et al.,2001] from the pRS315 plasmid. WT cells were grown O/N in selective SD (2% glucose, 0.7% Difco yeast nitrogen base without amino acids supplemented with the appropriate amino acids) media and refreshed in YPD media for 2 h
			Indirect	Western blot - wt vs TF mutant	Positive	CRM2268 (W303, mat a, chs5DELTA	Levels of Ena1-GFP from its endogenous locus after Indirect of the constitutively processed Rim101C [Lamb et al.,2001] from the pRS315 plasmid. DELTAchs5 cells were grown O/N in selective SD (2% glucose, 0.7% Difco yeast nitrogen base without amino acids supplemented with the appropriate amino acids) media and refreshed in YPD media for 2 h