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Regulation Reference(s)

The Yeastract team, is actively curating each regulation evidence code, association type and environmental condition.
Transcription
Factor		 Target
ORF/Genes		 References Evidence
Code		 Evidence
Experiment		 Association
Type		 Strain Environmental
Condition
Gln3p YKR039W PubMed external link Stanbrough M et al., Proc Natl Acad Sci U S A, 1995 Oct 10;92(21):9450-4Stanbrough M et al., Proc Natl Acad Sci U S A, 1995 Oct 10;92(21):9450-4 Indirect lacZ - wt vs TF mutant Positive S288c cells grown on glutamate as nitrogen source
PubMed external link Blinder D et al., J Bacteriol, 1995 Jul;177(14):4190-3Blinder D et al., J Bacteriol, 1995 Jul;177(14):4190-3 Direct DNA footprinting N/A S288c N/A
PubMed external link Stanbrough M et al., J Bacteriol, 1995 Jan;177(1):94-102Stanbrough M et al., J Bacteriol, 1995 Jan;177(1):94-102 Indirect lacZ - wt vs TF mutant Positive S288c glutamine/glutamate/urea as N source
PubMed external link Daugherty JR et al., J Bacteriol, 1993 Jan;175(1):64-73Daugherty JR et al., J Bacteriol, 1993 Jan;175(1):64-73 Indirect Northern blot - wt vs TF mutant Positive SIGMA1278b Wickerham's minimal medium + proline
PubMed external link Coffman JA et al., Mol Cell Biol, 1996 Mar;16(3):847-58Coffman JA et al., Mol Cell Biol, 1996 Mar;16(3):847-58 Indirect Northern blot - wt vs TF mutant Positive TCY1 Wickerham's minimal medium + proline
PubMed external link Stanbrough M et al., J Bacteriol, 1996 Apr;178(8):2465-8Stanbrough M et al., J Bacteriol, 1996 Apr;178(8):2465-8 Indirect lacZ - wt vs TF mutant Positive S288c glutamate as N source
PubMed external link Cox KH et al., Yeast, 1999 Jun 15;15(8):703-13Cox KH et al., Yeast, 1999 Jun 15;15(8):703-13 Indirect Microarray analysis - wt vs TF mutant Negative TCY1 Wickerham's minimal medium with 0,6% glucose and 0,1% of proline
PubMed external link Shamji AF et al., Curr Biol, 2000 Dec 14-28;10(24):1574-81Shamji AF et al., Curr Biol, 2000 Dec 14-28;10(24):1574-81 Indirect Microarray analysis - wt vs TF mutant Positive S288c 30 min 50 nM treatment with rapamycin in YPD
PubMed external link Rubio-Texeira M, FEBS Lett, 2007 Feb 6;581(3):541-50Rubio-Texeira M, FEBS Lett, 2007 Feb 6;581(3):541-50 Indirect lacZ - wt vs TF mutant Negative CKY835 Cells grown at 24 .C in minimal medium with ammonia as nitrogen source
Indirect lacZ - wt vs TF mutant Positive CKY835 Cells grown at 24 .C in minimal medium with glutamate as nitrogen source
Indirect lacZ - wt vs TF mutant Positive CKY835 Cells grown at 24 .C in minimal medium with glutamine as nitrogen source
PubMed external link Hirasaki M et al., Gene, 2008;():Hirasaki M et al., Gene, 2008;(): Indirect RT-PCR - wt vs TF mutant Positive S288c YPAD + 14mM Caffeine 1h
PubMed external link Georis I et al., Genetics, 2009 Mar;181(3):861-74Georis I et al., Genetics, 2009 Mar;181(3):861-74 Direct ChIP N/A TB123, FV018, FV036, FV041 YNB-glutamine medium treated with rapamycin (0.2 microg/ml) for 30 min
PubMed external link Georis I et al., Mol Cell Biol, 2009 Jul;29(13):3803-15Georis I et al., Mol Cell Biol, 2009 Jul;29(13):3803-15 Indirect RT-PCR - wt vs TF mutant Positive S288c Exponential-phase cells cultivated in synthetic growth medium containing 0.1% proline as nitrogen source
PubMed external link Staschke KA et al., J Biol Chem, 2010 May 28;285(22):16893-911Staschke KA et al., J Biol Chem, 2010 May 28;285(22):16893-911 Indirect Microarray analysis - wt vs TF mutant Positive EG328-1A RNA prepared from wt and DELTAgcn2DELTAgln3 cells treated for 1 h with 10 mM 3-AT
Indirect Microarray analysis - wt vs TF mutant Positive EG328-1A RNA prepared from wt and DELTAgcn2DELTAgln3 cells treated for 1 h with 200 nM Rapamycin
Indirect Microarray analysis - wt vs TF mutant Positive EG328-1A RNA prepared from wt and DELTAgcn4DELTAgln3 cells treated for 1 h with 200 nM Rapamycin
Indirect Microarray analysis - wt vs TF mutant Positive EG328-1A RNA prepared from wt and DELTAgln3 cells treated for 1 h with 200 nM Rapamycin
PubMed external link Fayyadkazan M et al., Microbiologyopen, 2014 Jun;3(3):271-87Fayyadkazan M et al., Microbiologyopen, 2014 Jun;3(3):271-87 Indirect RT-PCR - wt vs TF mutant Positive S288c cells grown in YPD medium and then treated with rapamycin o transferred to proline medium.
PubMed external link Chen X et al., J Biotechnol, 2017;():Chen X et al., J Biotechnol, 2017;(): Indirect RT-PCR - wt vs TF mutant Positive S288c YS58 strain with plasmid containing GLN3 ORF of MT2 or YS58 strains cloned into SalI and SacI of ADH1 promoter and terminator cloned into Escherichia coli-Yeast shuttle vector - Overexpression of GLN3; cells grown in synthetic complete medium
Indirect RT-PCR - wt vs TF mutant Positive YS58 wt and GLN3 (gene from MT2 strain) over-expressing strains were grown for 12, 24, 48 and 72 hours, in medium used for fermentation containing 30 g/L glucose, 0.5 g/L MgSO4·7H2O, 0.5 g/L KH2PO4, and 5 g/L L-phenylalanine (l-Phe), as a sole nitrogen source.
Indirect RT-PCR - wt vs TF mutant Positive YS58 wt and GLN3 (gene from YS58 strain) over-expressing strains were grown for 12, 24, 48 and 72 hours in medium used for fermentation containing 30 g/L glucose, 0.5 g/L MgSO4·7H2O, 0.5 g/L KH2PO4, and 5 g/L L-phenylalanine (l-Phe), as a sole nitrogen source.
PubMed external link Ponomarova O et al., Cell Syst, 2017 Oct 25;5(4):345-357.e6Ponomarova O et al., Cell Syst, 2017 Oct 25;5(4):345-357.e6 Indirect RNA-seq - wt vs TF mutant Negative S90 wt and DELTAgln3 cells were grown in YPAD medium
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