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Regulation Reference(s)

The Yeastract team, is actively curating each regulation evidence code, association type and environmental condition.
Transcription
Factor		 Target
ORF/Genes		 References Evidence
Code		 Evidence
Experiment		 Association
Type		 Strain Environmental
Condition
Aft2p YMR058W PubMed external link Blaiseau PL et al., J Biol Chem, 2001 Sep 7;276(36):34221-6Blaiseau PL et al., J Biol Chem, 2001 Sep 7;276(36):34221-6 Indirect Northern blot - wt vs TF mutant Positive CM3260 Iron depletion; cells in log phase grown in iron and copper limiting medium without iron addition
Indirect Northern blot - wt vs TF mutant Positive CM3260 Iron repletion; cells in log phase grown in iron and copper limiting medium supplemented with 100 microM iron
Indirect lacZ - wt vs TF mutant Positive CM3260 Iron depletion; cells in log phase grown in iron and copper limiting medium without iron addition
Indirect lacZ - wt vs TF mutant Positive CM3260 Iron repletion; cells in log phase grown in iron and copper limiting medium supplemented with 100 microM iron
PubMed external link Rutherford JC et al., Proc Natl Acad Sci U S A, 2001 Dec 4;98(25):14322-7Rutherford JC et al., Proc Natl Acad Sci U S A, 2001 Dec 4;98(25):14322-7 Direct EMSA N/A S288c in vitro
PubMed external link Courel M et al., Mol Cell Biol, 2005 Aug;25(15):6760-71Courel M et al., Mol Cell Biol, 2005 Aug;25(15):6760-71 Indirect Northern blot WT vs TF mutant Negative Y14438 Iron-depleted conditions - Defined medium (iron-limiting and copper-limiting yeast nitrogen base ); 30.C.
Indirect Northern blot WT vs TF mutant Positive Y14438 Iron- replete conditions - Defined medium (iron-limiting and copper-limiting yeast nitrogen base ) with 100uM ferric ammonium sulfate; 30.C.
Indirect Northern blot - wt vs TF mutant Positive SCMC01 Iron-depleted conditions - Defined medium (iron-limiting and copper-limiting yeast nitrogen base ); 30.C.
Indirect Northern blot - wt vs TF mutant Positive SCMC01 Iron- replete conditions - Defined medium (iron-limiting and copper-limiting yeast nitrogen base ) with 100uM ferric ammonium sulfate; 30.C.
Indirect Microarray analysis - wt vs TF mutant Positive SCMC01 Iron-depleted conditions - Defined medium (iron-limiting and copper-limiting yeast nitrogen base ); 30.C.
PubMed external link Dubacq C et al., Mol Genet Genomics, 2006 Feb;275(2):114-24Dubacq C et al., Mol Genet Genomics, 2006 Feb;275(2):114-24 Indirect Microarray analysis - wt vs TF mutant Positive S288c YPD
PubMed external link Gonçalves IR et al., Genetics, 2014 Jan;196(1):149-60Gonçalves IR et al., Genetics, 2014 Jan;196(1):149-60 Direct EMSA N/A S288c in vitro
PubMed external link Pérez-Sampietro M et al., Sci Rep, 2016;():Pérez-Sampietro M et al., Sci Rep, 2016;(): Indirect Northern blot - wt vs TF mutant Positive S288c 5h treatment with Sodium selenite
PubMed external link Pujol-Carrion N et al., Biochim Biophys Acta Mol Cell Res, 2021 Apr;1868(5):118974Pujol-Carrion N et al., Biochim Biophys Acta Mol Cell Res, 2021 Apr;1868(5):118974 Indirect lacZ - wt vs TF mutant Positive CML128 SD medium
Indirect lacZ - wt vs TF mutant Positive CML128 SD medium Fe depleted
Indirect lacZ - wt vs TF mutant Positive CML128 SD medium Fe repleted (10mM, 6h)
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