S.cerevisiae - Yeastract

Regulation Reference(s)

The Yeastract team, is actively curating each regulation evidence code, association type and environmental condition.

Transcription Factor	Target ORF/Genes	References	Evidence Code	Evidence Experiment	Association Type	Strain	Environmental Condition
Dal80p	YDL210W	PubMed Vissers S et al., Eur J Biochem, 1989 May 1;181(2):357-61	Indirect	Enzyme activity - wt vs TF mutant	Negative	SIGMA1278b	Medium + GABA
		PubMed Talibi D et al., Nucleic Acids Res, 1995 Feb 25;23(4):550-7	Indirect	lacZ - wt vs TF mutant	Negative	S288c	minimal proline medium (0,1% proline)
		PubMed AndrÃ© B et al., Nucleic Acids Res, 1995;():	Indirect	lacZ - wt vs TF mutant	Negative	S288c	proline (0,1%) as the sole source of nitrogen (minimal medium)
		PubMed Cunningham TS et al., J Bacteriol, 1994 Aug;176(15):4718-25	Direct	EMSA	N/A	S288c	in vitro
		PubMed Cunningham TS et al., J Bacteriol, 1993 Sep;175(18):5851-61	Direct	EMSA	N/A	S288c	in vitro
		PubMed Coffman JA et al., J Bacteriol, 1997 Jun;179(11):3416-29	Indirect	lacZ - wt vs TF mutant	Positive	TCY1	Minimal medium + proline
			Indirect	Northern blot - wt vs TF overexpression	Positive	TCY1	Minimal medium + proline
		PubMed Cox KH et al., Yeast, 1999 Jun 15;15(8):703-13	Indirect	Microarray analysis - wt vs TF mutant	Negative	TCY1	Wickerham's minimal medium with 0,6% glucose and 0,1% of proline
		PubMed Garcia SC et al., FEMS Microbiol Lett, 2000 Mar 15;184(2):219-24	Indirect	lacZ - wt vs TF mutant	Negative	S288c	Minimal proline medium 2 (MPM2 -in 1 l it contained 0.7 g MgSO4W7H2O, 1 g KH2PO4, 0.4 g CaCl2.2H20, 0.5 g NaCl, 1 g K2SO4, 10.5 g citric acidWH2O, 10 ml vitamin solution (250 mg D-biotin, 100 mg thiamineWHCl, 1 g myo-inositol, 200 mg calcium pantothenate and 100 mg pyridoxineWHCl per liter) and 1 ml of trace metal solution (10 mg H3BO3, 1 mg CuSO4W5H2O, 2 mg KI, 4 mg Na2MoO4W2H2O, 14 mg ZnSO4W7H2O
		PubMed Georis I et al., Mol Cell Biol, 2009 Jul;29(13):3803-15	Indirect	RT-PCR - wt vs TF mutant	Negative	S288c	Exponential-phase cells cultivated in synthetic growth medium containing 0.1% proline as nitrogen source
		PubMed Deed NK et al., Appl Microbiol Biotechnol, 2011 Mar;89(5):1537-49	Indirect	Microarray analysis - wt vs TF mutant	Negative	S288c	wine fermentation exponential phase
		PubMed Cardillo SB et al., Microbiology, 2012;():	Direct	ChIP	N/A	S288c	Minimal Media + GABA
		PubMed Levi CE et al., Biochem Biophys Res Commun, 2012 May 11;421(3):572-7	Direct	ChIP	N/A	S288c	Exponential phase cells cultivated in minimal growth medium containing acetate and GABA
		PubMed Palavecino-Ruiz M et al., Microbiology, 2017 Nov;163(11):1692-1701	Indirect	lacZ - wt vs TF mutant	Negative	23344c	Minimal medium containing a 0.17% Difco yeast nitrogen base (YNB without amino acids and ammonium sulphate), 2% glucose and 10mM proline.
			Indirect	lacZ - wt vs TF mutant	Negative	23344c	Minimal medium (0.17% Difco yeast nitrogen base, 2% glucose and 10mM proline) after incubation with 0.1mM GABA during 1h .
			Indirect	lacZ - wt vs TF mutant	Negative	23344c	wt and DELTAdal80 cells carrying the Yep-357-UGA4-lacZ plasmid were grown on 10mM proline and/or not incubated with 0.1mM GABA for 60 min
			Indirect	lacZ - wt vs TF mutant	Negative	23344c	wt and DELTAgzf3DELTAdal80 cells carrying the Yep-357-UGA4-lacZ plasmid were grown on 10mM proline and/or not incubated with 0.1mM GABA for 60 min
			Indirect	lacZ - wt vs TF mutant	Negative	23344c	wt and DELTAdal80DELTAleu3 cells carrying the Yep-357-UGA4-lacZ plasmid were grown on 10mM proline and/or not incubated with 0.1mM GABA for 60 min
			Indirect	lacZ - wt vs TF mutant	Negative	23344c	wt and DELTAgzf3DELTAdal80DELTAleu3 cells carrying the Yep-357-UGA4-lacZ plasmid were grown on 10mM proline and/or not incubated with 0.1mM GABA for 60 min
			Indirect	RT-PCR - wt vs TF mutant	N/A	23344c	Cells expressing HA-Dal80 were grown on proline without GABA vs incubated with 0.1 mM GABA for 30 min. ChIPs assays were carried out using antibodies against the HA epitope.