Transcription Factor |
Target ORF/Genes |
References |
Evidence Code |
Evidence Experiment |
Association Type |
Strain |
Environmental Condition |
Hap4p
|
YDR529C
|
PubMed
Blom J et al., Appl Environ Microbiol, 2000 May;66(5):1970-3Blom J et al., Appl Environ Microbiol, 2000 May;66(5):1970-3 |
Indirect |
Northern blot - wt vs TF overexpression |
Positive |
S288c |
YPD medium; mid-log phase |
PubMed
Lee TI et al., Science, 2002 Oct 25;298(5594):799-804Lee TI et al., Science, 2002 Oct 25;298(5594):799-804 |
Direct |
Chip-on-chip |
N/A |
S288c |
YPD medium; mid-log phase |
PubMed
Harbison CT et al., Nature, 2004 Sep 2;431(7004):99-104Harbison CT et al., Nature, 2004 Sep 2;431(7004):99-104 |
Direct |
Chip-on-chip |
N/A |
S288c |
YPD medium; mid-log phase |
PubMed
Chua G et al., Proc Natl Acad Sci U S A, 2006 Aug 8;103(32):12045-50Chua G et al., Proc Natl Acad Sci U S A, 2006 Aug 8;103(32):12045-50 |
Indirect |
Microarray analysis - wt vs TF overexpression |
Positive |
N/A |
Cells grown in selective medium supplemented with 2% raffinose before induction with 2% galactose for 3 h |
PubMed
Dueñas-Sánchez R et al., Int J Food Microbiol, 2010;():Dueñas-Sánchez R et al., Int J Food Microbiol, 2010;(): |
Direct |
RT-PCR - wt vs mutant promoter sequence |
Positive |
S288c |
Yeasts were cultivated in Beet molasses diluted to 3.6% sucrose and supplemented with biotin (0.5 mg/l) and diammonium phosphate (0.5 g/l) (MAB medium) |
PubMed
Galdieri L et al., Mol Cell Biol, 2016 Jan 19;36(7):1064-77Galdieri L et al., Mol Cell Biol, 2016 Jan 19;36(7):1064-77 |
Indirect |
RT-PCR - wt vs TF mutant |
Positive |
S288c |
Cells were grown at 28.C in YEP medium (1% yeast extract, 2% Bacto peptone) containing 5% glucose |
PubMed
Zhang T et al., J Biol Chem, 2017 Oct 13;292(41):16942-16954Zhang T et al., J Biol Chem, 2017 Oct 13;292(41):16942-16954 |
Direct |
ChIP |
N/A |
W303 |
YEP medium |
PubMed
Holland P et al., Nucleic Acids Res, 2019 Jun 4;47(10):4986-5000Holland P et al., Nucleic Acids Res, 2019 Jun 4;47(10):4986-5000 |
Direct |
ChIP-exo |
N/A |
CEN.PK 113-5D |
Cells were cultivated in chemostats in ethanol limited medium with a dilution rate of 0.1 h-1 at 30.C. Samples were collected after steady state was achieved for 48-60 h |
Direct |
ChIP-exo |
N/A |
CEN.PK 113-5D |
Cells were cultivated in chemostats in respiratory glucose limited medium with a dilution rate of 0.1 h-1 at 30.C. Samples were collected after steady state was achieved for 48-60 h |