PubMed
Chua G et al., Proc Natl Acad Sci U S A, 2006 Aug 8;103(32):12045-50Chua G et al., Proc Natl Acad Sci U S A, 2006 Aug 8;103(32):12045-50
Indirect
Microarray analysis - wt vs TF overexpression
Negative
N/A
Cells grown in selective medium supplemented with 2% raffinose before induction with 2% galactose for 3 h
PubMed
Dueñas-Sánchez R et al., Int J Food Microbiol, 2010;():Dueñas-Sánchez R et al., Int J Food Microbiol, 2010;():
Direct
RT-PCR - wt vs mutant promoter sequence
Positive
S288c
Yeasts were cultivated in Beet molasses diluted to 3.6% sucrose and supplemented with biotin (0.5 mg/l) and diammonium phosphate (0.5 g/l) (MAB medium)