PubMed
Blom J et al., Appl Environ Microbiol, 2000 May;66(5):1970-3Blom J et al., Appl Environ Microbiol, 2000 May;66(5):1970-3
Indirect
Northern blot - wt vs TF overexpression
Positive
S288c
YPD medium; mid-log phase
PubMed
Chua G et al., Proc Natl Acad Sci U S A, 2006 Aug 8;103(32):12045-50Chua G et al., Proc Natl Acad Sci U S A, 2006 Aug 8;103(32):12045-50
Indirect
Microarray analysis - wt vs TF overexpression
Positive
N/A
Cells grown in selective medium supplemented with 2% raffinose before induction with 2% galactose for 3 h
PubMed
Schuurmans JM et al., FEMS Yeast Res, 2008 Feb;8(1):26-34Schuurmans JM et al., FEMS Yeast Res, 2008 Feb;8(1):26-34
Indirect
Microarray analysis - wt vs TF mutant
Positive
CEN.PK113-7D
wt versus Tf overexpression 0.67 %YNB, 2% glucose and 0.1 M phtalic acid pH 5 30.C mid exp cells
PubMed
Dueñas-Sánchez R et al., Int J Food Microbiol, 2010;():Dueñas-Sánchez R et al., Int J Food Microbiol, 2010;():
Direct
RT-PCR - wt vs mutant promoter sequence
Positive
S288c
Yeasts were cultivated in Beet molasses diluted to 3.6% sucrose and supplemented with biotin (0.5 mg/l) and diammonium phosphate (0.5 g/l) (MAB medium)