Transcription Factor |
Target ORF/Genes |
References |
Evidence Code |
Evidence Experiment |
Association Type |
Strain |
Environmental Condition |
Hsf1p
|
YAL005C
|
PubMed
Smith BJ et al., Mol Cell Biol, 1991 May;11(5):2647-55Smith BJ et al., Mol Cell Biol, 1991 May;11(5):2647-55 |
Indirect |
Northern blot - wt vs TF mutant |
Positive |
S288c |
Cells were grown in SD medium at 23°C. Cells were harvested 30 min after a shift to 37°C |
PubMed
Halladay JT et al., Mol Cell Biol, 1995 Sep;15(9):4890-7Halladay JT et al., Mol Cell Biol, 1995 Sep;15(9):4890-7 |
Direct |
EMSA |
N/A |
S288c |
in vitro |
PubMed
Young MR et al., Mol Cell Biol, 1993 Sep;13(9):5637-46Young MR et al., Mol Cell Biol, 1993 Sep;13(9):5637-46 |
Direct |
EMSA |
N/A |
S288c |
in vitro |
PubMed
Boy-Marcotte E et al., Mol Microbiol, 1999 Jul;33(2):274-83Boy-Marcotte E et al., Mol Microbiol, 1999 Jul;33(2):274-83 |
Indirect |
Proteomics - wt vs TF mutant |
Positive |
MYY290 |
Heat-shock: Exponential-phase cells shifted from 25C to 38C |
PubMed
Lee TI et al., Science, 2002 Oct 25;298(5594):799-804Lee TI et al., Science, 2002 Oct 25;298(5594):799-804 |
Direct |
Chip-on-chip |
N/A |
S288c |
YPD medium; mid-log phase |
PubMed
Harbison CT et al., Nature, 2004 Sep 2;431(7004):99-104Harbison CT et al., Nature, 2004 Sep 2;431(7004):99-104 |
Direct |
Chip-on-chip |
N/A |
S288c |
N/A |
PubMed
Yamamoto A et al., J Biol Chem, 2005 Mar 25;280(12):11911-9Yamamoto A et al., J Biol Chem, 2005 Mar 25;280(12):11911-9 |
Indirect |
Northern blot - wt vs TF mutant |
Positive |
S288c |
YPD medium 39ºC (heat shock) |
PubMed
Chua G et al., Proc Natl Acad Sci U S A, 2006 Aug 8;103(32):12045-50Chua G et al., Proc Natl Acad Sci U S A, 2006 Aug 8;103(32):12045-50 |
Indirect |
Microarray analysis - wt vs TF overexpression |
Positive |
N/A |
Cells grown in selective medium supplemented with 2% raffinose before induction with 2% galactose for 3 h |
PubMed
Hou J et al., FEMS Yeast Res, 2014 May;14(3):481-94Hou J et al., FEMS Yeast Res, 2014 May;14(3):481-94 |
Indirect |
Microarray analysis - wt vs TF overexpression |
Positive |
S288c |
DTT inhibition |
PubMed
SolÃs EJ et al., Mol Cell, 2016 Jul 7;63(1):60-71SolÃs EJ et al., Mol Cell, 2016 Jul 7;63(1):60-71 |
Indirect |
Net-seq |
Positive |
S288c |
basal conditions |
PubMed
de Jonge WJ et al., EMBO J, 2017 Feb 1;36(3):274-290de Jonge WJ et al., EMBO J, 2017 Feb 1;36(3):274-290 |
Direct |
ChIP |
N/A |
S288C |
SC medium |
Indirect |
RT-PCR - wt vs TF mutant |
Negative |
S288C |
SC medium |
PubMed
Krakowiak J et al., Elife, 2018 Feb 2;7(0):e31668Krakowiak J et al., Elife, 2018 Feb 2;7(0):e31668 |
Indirect |
Western blot - wt vs TF mutant |
Positive |
W303 |
SDC media |
PubMed
Pincus D et al., Mol Biol Cell, 2018 Dec 15;29(26):3168-3182Pincus D et al., Mol Biol Cell, 2018 Dec 15;29(26):3168-3182 |
Direct |
ChIP-seq |
N/A |
BY4741 |
YPDA (with 0.002% adenine), non-heat shock, 5´heat shock and 120´heat shock (39.C)) |
Direct |
ChIP-seq |
N/A |
BY4741 |
YPDA (with 0.002% adenine) Rapamycin (1 microM), non-heat shock |
Direct |
ChIP-seq |
N/A |
BY4741 |
YPDA (with 0.002% adenine), 5' heat shock |
Indirect |
NAC-seq (nascent mRNA-sequencing) |
Positive |
BY4741 |
YPDA (with 0.002% adenine) Rapamycin (1 microM), non-heat shock |
Indirect |
NAC-seq (nascent mRNA-sequencing) |
Positive |
BY4741 |
YPDA (with 0.002% adenine), 5' heat shock |
PubMed
Rossi MJ et al., Nature, 2021 Apr;592(7853):309-314Rossi MJ et al., Nature, 2021 Apr;592(7853):309-314 |
Direct |
ChIP-exo |
N/A |
BY4741 |
YPD medium, 25.C |