Transcription Factor |
Target ORF/Genes |
References |
Evidence Code |
Evidence Experiment |
Association Type |
Strain |
Environmental Condition |
Hsf1p
|
YBL075C
|
PubMed
Halladay JT et al., Mol Cell Biol, 1995 Sep;15(9):4890-7Halladay JT et al., Mol Cell Biol, 1995 Sep;15(9):4890-7 |
Indirect |
Western blot - wt vs TF mutant |
Positive |
S288c |
growth at 23 and 34ºC; ?ssa1/2 background with low activity allele |
PubMed
Young MR et al., Mol Cell Biol, 1993 Sep;13(9):5637-46Young MR et al., Mol Cell Biol, 1993 Sep;13(9):5637-46 |
Direct |
lacZ - wt vs mutant promoter sequence |
Positive |
S288c |
heat shock (cells grown to mid log phase at 23ºC and transfered to 37ºC for 15 min) |
PubMed
Treger JM et al., J Biol Chem, 1998 Oct 9;273(41):26875-9Treger JM et al., J Biol Chem, 1998 Oct 9;273(41):26875-9 |
Indirect |
Northern blot - wt vs TF mutant |
Positive |
JT659 |
Heat-shock treatment |
PubMed
Boy-Marcotte E et al., Mol Microbiol, 1999 Jul;33(2):274-83Boy-Marcotte E et al., Mol Microbiol, 1999 Jul;33(2):274-83 |
Indirect |
Proteomics - wt vs TF mutant |
Positive |
MYY290 |
Heat-shock: Exponential-phase cells shifted from 25C to 38C |
PubMed
Harbison CT et al., Nature, 2004 Sep 2;431(7004):99-104Harbison CT et al., Nature, 2004 Sep 2;431(7004):99-104 |
Direct |
Chip-on-chip |
N/A |
S288c |
N/A |
PubMed
Yamamoto A et al., J Biol Chem, 2005 Mar 25;280(12):11911-9Yamamoto A et al., J Biol Chem, 2005 Mar 25;280(12):11911-9 |
Indirect |
Northern blot - wt vs TF mutant |
Positive |
S288c |
YPD medium 39ºC (heat shock) |
PubMed
Chua G et al., Proc Natl Acad Sci U S A, 2006 Aug 8;103(32):12045-50Chua G et al., Proc Natl Acad Sci U S A, 2006 Aug 8;103(32):12045-50 |
Indirect |
Microarray analysis - wt vs TF overexpression |
Positive |
N/A |
Cells grown in selective medium supplemented with 2% raffinose before induction with 2% galactose for 3 h |
PubMed
Eastmond DL et al., J Biol Chem, 2006 Oct 27;281(43):32909-21Eastmond DL et al., J Biol Chem, 2006 Oct 27;281(43):32909-21 |
Indirect |
Microarray analysis - wt vs TF mutant |
Positive |
W303-1A |
Cells grown in YPAD at 30.C to mid-log phase (A600 of 0.5) submitted to heat-shock (37.C, 15 min) |
PubMed
Yamamoto A et al., Eukaryot Cell, 2007 Aug;6(8):1373-9Yamamoto A et al., Eukaryot Cell, 2007 Aug;6(8):1373-9 |
Indirect |
RT-PCR - wt vs TF mutant |
Positive |
S288c |
CDNB |
PubMed
Han Q et al., Biochem J, 2008 Feb 1;409(3):779-88Han Q et al., Biochem J, 2008 Feb 1;409(3):779-88 |
Direct |
ChIP |
N/A |
S288c |
Heat shock |
PubMed
Bandhakavi S et al., PLoS One, 2008;():Bandhakavi S et al., PLoS One, 2008;(): |
Indirect |
RT-PCR - wt vs TF mutant |
Positive |
n/a |
wt versus TF overexpression YPD media 25.C mid exp cells |
PubMed
Wang S et al., Nucleic Acids Res, 2010 Aug;38(15):e154Wang S et al., Nucleic Acids Res, 2010 Aug;38(15):e154 |
Indirect |
RT-PCR - wt vs TF overexpression |
Positive |
S288c |
Sgal-Ura_His media + Heat Shock |
PubMed
Hou J et al., FEMS Yeast Res, 2014 May;14(3):481-94Hou J et al., FEMS Yeast Res, 2014 May;14(3):481-94 |
Indirect |
Microarray analysis - wt vs TF overexpression |
Positive |
S288c |
DTT inhibition |