Transcription Factor |
Target ORF/Genes |
References |
Evidence Code |
Evidence Experiment |
Association Type |
Strain |
Environmental Condition |
Hsf1p
|
YER103W
|
PubMed
Boorstein WR et al., J Biol Chem, 1990 Nov 5;265(31):18912-21Boorstein WR et al., J Biol Chem, 1990 Nov 5;265(31):18912-21 |
N/A |
|
N/A |
S288c |
N/A |
PubMed
Halladay JT et al., Mol Cell Biol, 1995 Sep;15(9):4890-7Halladay JT et al., Mol Cell Biol, 1995 Sep;15(9):4890-7 |
Direct |
EMSA |
N/A |
S288c |
in vitro |
PubMed
Young MR et al., Mol Cell Biol, 1993 Sep;13(9):5637-46Young MR et al., Mol Cell Biol, 1993 Sep;13(9):5637-46 |
Direct |
EMSA |
N/A |
S288c |
in vitro |
PubMed
Boy-Marcotte E et al., Mol Microbiol, 1999 Jul;33(2):274-83Boy-Marcotte E et al., Mol Microbiol, 1999 Jul;33(2):274-83 |
Indirect |
Proteomics - wt vs TF mutant |
Positive |
MYY290 |
Heat-shock: Exponential-phase cells shifted from 25C to 38C |
PubMed
Harbison CT et al., Nature, 2004 Sep 2;431(7004):99-104Harbison CT et al., Nature, 2004 Sep 2;431(7004):99-104 |
Direct |
Chip-on-chip |
N/A |
S288c |
N/A |
PubMed
Yamamoto A et al., J Biol Chem, 2005 Mar 25;280(12):11911-9Yamamoto A et al., J Biol Chem, 2005 Mar 25;280(12):11911-9 |
Indirect |
Northern blot - wt vs TF mutant |
Positive |
S288c |
YPD medium 39ºC (heat shock) |
PubMed
Eastmond DL et al., J Biol Chem, 2006 Oct 27;281(43):32909-21Eastmond DL et al., J Biol Chem, 2006 Oct 27;281(43):32909-21 |
Indirect |
Microarray analysis - wt vs TF mutant |
Positive |
W303-1A |
Cells grown in YPAD at 30.C to mid-log phase (A600 of 0.5) submitted to heat-shock (37.C, 15 min) |
PubMed
Han Q et al., Biochem J, 2008 Feb 1;409(3):779-88Han Q et al., Biochem J, 2008 Feb 1;409(3):779-88 |
Direct |
ChIP |
N/A |
S288c |
Heat shock |
PubMed
Erkina TY et al., Mol Cell Biol, 2008 Feb;28(4):1207-17Erkina TY et al., Mol Cell Biol, 2008 Feb;28(4):1207-17 |
Direct |
ChIP |
N/A |
S288c |
N/A |
PubMed
Bandhakavi S et al., PLoS One, 2008;():Bandhakavi S et al., PLoS One, 2008;(): |
Indirect |
RT-PCR - wt vs TF mutant |
Positive |
n/a |
wt versus TF overexpression YPD media 25.C mid exp cells |
PubMed
Hou J et al., FEMS Yeast Res, 2014 May;14(3):481-94Hou J et al., FEMS Yeast Res, 2014 May;14(3):481-94 |
Indirect |
Microarray analysis - wt vs TF overexpression |
Positive |
S288c |
DTT inhibition |
PubMed
Pincus D et al., Mol Biol Cell, 2018 Dec 15;29(26):3168-3182Pincus D et al., Mol Biol Cell, 2018 Dec 15;29(26):3168-3182 |
Direct |
ChIP-seq |
N/A |
BY4741 |
YPDA (with 0.002% adenine), non-heat shock, 5´heat shock and 120´heat shock |
Direct |
ChIP-seq |
N/A |
BY4741 |
YPDA (with 0.002% adenine), 5' heat shock |
Indirect |
NAC-seq (nascent mRNA-sequencing) |
Positive |
BY4741 |
YPDA (with 0.002% adenine), 5' heat shock |