Transcription Factor |
Target ORF/Genes |
References |
Evidence Code |
Evidence Experiment |
Association Type |
Strain |
Environmental Condition |
Hsf1p
|
YFL014W
|
PubMed
Treger JM et al., J Biol Chem, 1998 Oct 9;273(41):26875-9Treger JM et al., J Biol Chem, 1998 Oct 9;273(41):26875-9 |
Indirect |
Northern blot - wt vs TF mutant |
Positive |
JT659 |
Heat-shock treatment |
PubMed
Boy-Marcotte E et al., Mol Microbiol, 1999 Jul;33(2):274-83Boy-Marcotte E et al., Mol Microbiol, 1999 Jul;33(2):274-83 |
Indirect |
Proteomics - wt vs TF mutant |
Positive |
MYY290 |
Heat-shock: Exponential-phase cells shifted from 25C to 38C |
PubMed
Yamamoto A et al., J Biol Chem, 2005 Mar 25;280(12):11911-9Yamamoto A et al., J Biol Chem, 2005 Mar 25;280(12):11911-9 |
Indirect |
Microarray analysis - wt vs TF mutant |
Positive |
S288c |
YPD medium 39ÂșC (heat shock) |
PubMed
Workman CT et al., Science, 2006 May 19;312(5776):1054-9Workman CT et al., Science, 2006 May 19;312(5776):1054-9 |
Direct |
Chip-on-chip |
N/A |
S288c |
N/A |
PubMed
Chua G et al., Proc Natl Acad Sci U S A, 2006 Aug 8;103(32):12045-50Chua G et al., Proc Natl Acad Sci U S A, 2006 Aug 8;103(32):12045-50 |
Indirect |
Microarray analysis - wt vs TF overexpression |
Positive |
N/A |
Cells grown in selective medium supplemented with 2% raffinose before induction with 2% galactose for 3 h |
PubMed
Eastmond DL et al., J Biol Chem, 2006 Oct 27;281(43):32909-21Eastmond DL et al., J Biol Chem, 2006 Oct 27;281(43):32909-21 |
Indirect |
Microarray analysis - wt vs TF mutant |
Positive |
W303-1A |
Cells grown in YPAD at 30.C to mid-log phase (A600 of 0.5) submitted to heat-shock (37.C, 15 min) |
PubMed
Erkina TY et al., Mol Cell Biol, 2008 Feb;28(4):1207-17Erkina TY et al., Mol Cell Biol, 2008 Feb;28(4):1207-17 |
Direct |
ChIP |
N/A |
S288c |
N/A |
PubMed
Wang S et al., Nucleic Acids Res, 2010 Aug;38(15):e154Wang S et al., Nucleic Acids Res, 2010 Aug;38(15):e154 |
Indirect |
RT-PCR - wt vs TF overexpression |
Positive |
S288c |
Sgal-Ura_His media + Heat Shock |
PubMed
Chowdhary S et al., Cell Rep, 2019 Jan 2;26(1):18-28.e5Chowdhary S et al., Cell Rep, 2019 Jan 2;26(1):18-28.e5 |
Direct |
ChIP |
N/A |
BY4741 |
YPDA heat-shocked, subjected to 39.C thermal upshift (10min) |
PubMed
Rossi MJ et al., Nature, 2021 Apr;592(7853):309-314Rossi MJ et al., Nature, 2021 Apr;592(7853):309-314 |
Direct |
ChIP-exo |
N/A |
BY4741 |
YPD medium, 25.C |