S.cerevisiae - Yeastract

Regulation Reference(s)

The Yeastract team, is actively curating each regulation evidence code, association type and environmental condition.

Transcription Factor	Target ORF/Genes	References	Evidence Code	Evidence Experiment	Association Type	Strain	Environmental Condition
Hsf1p	YLR216C	PubMed Liu XD et al., J Biol Chem, 1999 Sep 17;274(38):26654-60	Indirect	Northern blot - wt vs TF mutant	Positive	S288c	Heat shock (41ºC for 16 min in SC-Met-Cys medium )
		PubMed Lee TI et al., Science, 2002 Oct 25;298(5594):799-804	Direct	Chip-on-chip	N/A	S288c	YPD medium; mid-log phase
		PubMed Harbison CT et al., Nature, 2004 Sep 2;431(7004):99-104	Direct	Chip-on-chip	N/A	S288c	N/A
		PubMed Yamamoto A et al., J Biol Chem, 2005 Mar 25;280(12):11911-9	Indirect	Microarray analysis - wt vs TF mutant	Positive	S288c	YPD medium 39ºC (heat shock)
		PubMed Imazu H et al., Eukaryot Cell, 2005 Jun;4(6):1050-6	Indirect	RT-PCR - wt vs TF mutant	Positive	HS126	Cells were grown in ESD medium lacking uracil at 28.C, and then the temperature was shifted to 39.C.
		PubMed Workman CT et al., Science, 2006 May 19;312(5776):1054-9	Direct	Chip-on-chip	N/A	S288c	N/A
		PubMed Eastmond DL et al., J Biol Chem, 2006 Oct 27;281(43):32909-21	Indirect	Microarray analysis - wt vs TF mutant	Positive	W303-1A	Cells grown in YPAD at 30.C to mid-log phase (A600 of 0.5) submitted to heat-shock (37.C, 15 min)
		PubMed Yamamoto N et al., Eukaryot Cell, 2008 May;7(5):783-90	Indirect	RT-PCR - wt vs TF mutant	Positive	HS170T	wt versus hsf1 R206S-H220R ( arginine to serine at residue 206 and histidine to arginine at residue 220) ESD media lacking uracil heat shock(temperature shift from 28.C to 37.C for 1h and then 48.C
		PubMed Reimand J et al., Nucleic Acids Res, 2010 Aug;38(14):4768-77	Indirect	Microarray analysis - wt vs TF mutant	Positive	S288c	YPD medium; mid-log phase
		PubMed SolÃs EJ et al., Mol Cell, 2016 Jul 7;63(1):60-71	Indirect	Net-seq	Positive	S288c	basal conditions
		PubMed de Jonge WJ et al., EMBO J, 2017 Feb 1;36(3):274-290	Direct	ChIP	N/A	S288C	SC medium
		PubMed de Jonge WJ et al., EMBO J, 2017 Feb 1;36(3):274-290	Indirect	RT-PCR - wt vs TF mutant	Negative	S288C	SC medium
		PubMed Pincus D et al., Mol Biol Cell, 2018 Dec 15;29(26):3168-3182	Direct	ChIP-seq	N/A	BY4741	YPDA (with 0.002% adenine), non-heat shock, 5Â´heat shock and 120Â´heat shock
			Direct	ChIP-seq	N/A	BY4741	YPDA (with 0.002% adenine) Rapamycin (1 microM), non-heat shock
			Direct	ChIP-seq	N/A	BY4741	YPDA (with 0.002% adenine), 5' heat shock
			Indirect	NAC-seq (nascent mRNA-sequencing)	Positive	BY4741	YPDA (with 0.002% adenine) Rapamycin (1 microM), non-heat shock
			Indirect	NAC-seq (nascent mRNA-sequencing)	Positive	BY4741	YPDA (with 0.002% adenine), 5' heat shock
		PubMed Rossi MJ et al., Nature, 2021 Apr;592(7853):309-314	Direct	ChIP-exo	N/A	BY4741	YPD medium, 25.C