S.cerevisiae - Yeastract

Regulation Reference(s)

The Yeastract team, is actively curating each regulation evidence code, association type and environmental condition.

Transcription Factor	Target ORF/Genes	References	Evidence Code	Evidence Experiment	Association Type	Strain	Environmental Condition
Met32p	YLR303W	PubMed Blaiseau PL et al., Mol Cell Biol, 1997 Jul;17(7):3640-8	Indirect	Northern blot - wt vs TF mutant	Positive	S288c	medium without metionine for 80min
		PubMed Aranda A et al., Appl Environ Microbiol, 2004 Apr;70(4):1913-22	Indirect	Western blot - wt vs TF mutant	Positive	S288c	1 g/L acetaldehyde (1H)
			Indirect	Western blot - wt vs TF mutant	Positive	S288c	log-phase growth; 2 % glucose YPD
		PubMed Harbison CT et al., Nature, 2004 Sep 2;431(7004):99-104	Direct	Chip-on-chip	N/A	S288c	SC medium; mid-log phase
		PubMed Su NY et al., J Biol Chem, 2008 Apr 25;283(17):11615-24	Indirect	RT-PCR - wt vs TF mutant	Positive	BF264-15D	met31met32DELTA double deletion mutant vs met32DELTA single mutant SC media 30.C lacking methionine
		PubMed Pereira Y et al., Toxicol Sci, 2008 Dec;106(2):400-12	Indirect	Microarray analysis - wt vs TF mutant	Negative	YPH98	135 min at 30.C in YNB medium + 20microM chromate
		PubMed Moxley JF et al., Proc Natl Acad Sci U S A, 2009 Apr 21;106(16):6477-82	Indirect	Microarray analysis - wt vs TF mutant	Positive	BY4741	YNB media
		PubMed Lee TA et al., Mol Biol Cell, 2010 Feb 1;21(3):456-69	Indirect	Microarray analysis - wt vs TF mutant	Negative	W303 (met4::GAL-MET4 met30DELTA met31DELTA met32DELTA)	Shift from YEP + 2% raffinose to YEP + 2-3% galactose
		PubMed Cormier L et al., Nucleic Acids Res, 2010 Aug;38(15):4998-5014	Direct	ChIP	N/A	W303	Isogenic strain expressing Myc-tagged version of Met32 was grown to early log phase in YPD medium and exposed to 0.5mM Cd2+.
		PubMed Carrillo E et al., Mol Biol Cell, 2012 May;23(10):1928-42	Direct	ChIP	N/A	W303	90 min of Met4 induction
			Direct	Chip-on-chip	N/A	W303	90 min of Met4 induction
		PubMed Ndoja A et al., Mol Cell, 2014 Mar 20;53(6):893-903	Direct	ChIP	N/A	S288c	Met32 was C-terminally tagged with 13xMyc at the endogenous MET32 locus in the ufd1-1 strain. This strain was grown in YPD at 25 .C to log phase and shifted to 37 .C for 30 min. Met32 occupancy at the MET17 promoter was monitored by ChIP with anti-Myc antibodies