Yeastract
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Regulation Reference(s)

The Yeastract team, is actively curating each regulation evidence code, association type and environmental condition.
Transcription
Factor
Target
ORF/Genes
References Evidence
Code
Evidence
Experiment
Association
Type
Strain Environmental
Condition
Pho4p YCR098C PubMed external link Almaguer C et al., Eukaryot Cell, 2003 Aug;2(4):729-36Almaguer C et al., Eukaryot Cell, 2003 Aug;2(4):729-36 Indirect Enzyme activity - wt vs TF mutant Positive S288c High Pi - Strains were pregrown in high-Pi, I+ medium were harvested and used to inoculate the following media: high Pi, I?; and high Pi, I+. Following three to five generations of growth, lysates were obtained by glass bead breakage
PubMed external link Almaguer C et al., J Biol Chem, 2004 Jul 23;279(30):31937-42Almaguer C et al., J Biol Chem, 2004 Jul 23;279(30):31937-42 Indirect CAT - wt vs mutant promoter sequence Positive S288c 75mM inositol 0,2mM KH2PO4
PubMed external link Harbison CT et al., Nature, 2004 Sep 2;431(7004):99-104Harbison CT et al., Nature, 2004 Sep 2;431(7004):99-104 Direct Chip-on-chip N/A S288c N/A
PubMed external link Chua G et al., Proc Natl Acad Sci U S A, 2006 Aug 8;103(32):12045-50Chua G et al., Proc Natl Acad Sci U S A, 2006 Aug 8;103(32):12045-50 Indirect Microarray analysis - wt vs TF overexpression Positive N/A Cells grown in selective medium supplemented with 2% raffinose before induction with 2% galactose for 3 h
PubMed external link Nishizawa M et al., PLoS Biol, 2008 Dec 23;6(12):2817-30Nishizawa M et al., PLoS Biol, 2008 Dec 23;6(12):2817-30 Direct Chip-on-chip N/A S288c YNB medium without phosphate + 0,2microM (low Pi; phosphate limitation)
PubMed external link He et al., Elife, 2017;6(0):e25157He et al., Elife, 2017;6(0):e25157 Indirect RNA-seq - wt vs TF mutant Positive EY0057 pho80DELTA EY0057 pho80DELTA; Minimal medium; mid-log phase
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