PubMed
Harbison CT et al., Nature, 2004 Sep 2;431(7004):99-104Harbison CT et al., Nature, 2004 Sep 2;431(7004):99-104
Direct
Chip-on-chip
N/A
S288c
Unstressed log-phase growth (control)
SC medium; mid-log phase
N/A
PubMed
Subramanian M et al., Molecular microbiology, 2005 Jul;57(1):53-69Subramanian M et al., Molecular microbiology, 2005 Jul;57(1):53-69
Indirect
Northern blot - wt vs TF mutant
Negative
S288c
Nitrogen source quality/availability
Minimal B medium upon glycine removal
N/A
PubMed
Yi DG et al., FEBS letters, 2015 Aug 19;589(18):2409-16Yi DG et al., FEBS letters, 2015 Aug 19;589(18):2409-16
Indirect
RNA-seq analysis - WT vs TF Deletion
Positive
SK1
Unstressed log-phase growth (control)
Cells from a stationary culture in YPD medium were inoculated into fresh YPD medium to a final concentration equivalent to an A 600 of 0.25, and grown at 30 °C for 12 h.
1.03219589667179
Indirect
RNA-seq analysis - WT vs TF Deletion
Positive
SK1
Cell cycle/morphology
Cells were grown in YPA for 13.5–14 hr at 30°, harvested, resuspended in 2% potassium acetate, and sporulated at 30°. Then, 15 OD600 units of cells were collected at 4 hr in meiosis for RNAseq. Transcription factor gene deletion.
