PubMed
Iwai K et al., The Journal of biological chemistry, 2010 Apr 2;285(14):10597-604Iwai K et al., The Journal of biological chemistry, 2010 Apr 2;285(14):10597-604
Indirect
lacZ - wt vs TF mutant
Negative
BY4742
Ion/metal/phosphate/sulfur/vitamin availability
RTG3-cat reporter gene was transformed into a wt vs isogenic bHLH knock-out RO+ strains (DELTAino4 with mitochondrial DNA). CIT2-lacZ expression was assayed from cultures grown in the absence (I-C-) or presence of inositol and choline (I+C+) containing either low-phosphate (Pi low) or high-phosphate (Pi high). The I-C- medium contained 10 microM inositol to allow for growth of this inositol auxotrophs