PubMed
Lardenois A et al., Molecular genetics and genomics : MGG, 2015 Oct;290(5):2031-46Lardenois A et al., Molecular genetics and genomics : MGG, 2015 Oct;290(5):2031-46
Indirect
Microarray analysis - WT vs TF Deletion
Negative
SK1
Grown in sporulation medium (SPII for 10 h) under standard conditions. Transcription factor gene deletion.
-2.62352
Indirect
Microarray analysis - WT vs TF Deletion
Negative
SK1
Grown in sporulation medium (SPII for 8 h) under standard conditions. Transcription factor gene deletion.
-2.726505
Indirect
Microarray analysis - WT vs TF Deletion
Negative
SK1
Grown in rich media with glucose (YPD) under standard conditions. Transcription factor gene deletion.
Grown overnight at 30 °C in phosphate-free YNB medium supplemented with 7.2 mM potassium phosphate (pH 5.8), 20 mg/L histidine, leucine, methionine, and uracil, and 50 mM KCl, then used to inoculate 50 mL cultures at OD620 = 0.1 and grown at 30 °C, 180 rpm to OD620 = 0.5. Transcription factor gene deletion.
-0.495194714624007
PubMed
Harris A et al., Genetics, 2023 Oct 4;225(2):Harris A et al., Genetics, 2023 Oct 4;225(2):
Indirect
RNA-seq analysis - WT vs TF Under-expression
Negative
SK1
Meiotic 2h: Cells were transitioning from premeiotic starvation into meiosis. They were grown in YPD for ~24 h to saturation, then inoculated into BYTA (1% yeast extract, 2% bacto tryptone, 1% potassium acetate, 50 mM potassium phthalate) at OD600 = 0.25 and grown 16–18 h at 30 °C to OD600 ≥ 5. Cells were then pelleted, washed with sterile water, and resuspended in SPM (2% KOAc, 0.02% raffinose, amino acid supplements, pH 7) at OD600 = 1.85, shaken at 30 °C and 275 rpm. At 0.5 h in SPM, β-estradiol (5 nM) and auxin (200 µM) were added simultaneously, followed by CuSO4 (50 µM) at 2 h to trigger IME1/4 expression and meiotic entry. Sampled at 2 h.
