Yeastract
YEASTRACT+ INESC-ID IST IBB
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Regulation Reference(s)

The Yeastract team, is actively curating each regulation evidence code, association type and environmental condition.
Transcription
Factor		 Target
ORF/Genes		 References Evidence
Code		 Evidence
Experiment		 Association
Type		 Strain Environmental
Group		 Environmental
Condition		 Log2FC
Dal81p YBR068C PubMed external link Bernard F et al., Molecular microbiology, 2001 Jul;41(2):489-502Bernard F et al., Molecular microbiology, 2001 Jul;41(2):489-502 Indirect lacZ - wt vs TF mutant Positive S288c Unstressed log-phase growth (control) amino acid induction N/A
PubMed external link Lee TI et al., Science (New York, N.Y.), 2002 Oct 25;298(5594):799-804Lee TI et al., Science (New York, N.Y.), 2002 Oct 25;298(5594):799-804 Direct Chip-on-chip N/A S288c Unstressed log-phase growth (control) YPD medium; mid-log phase N/A
PubMed external link Harbison CT et al., Nature, 2004 Sep 2;431(7004):99-104Harbison CT et al., Nature, 2004 Sep 2;431(7004):99-104 Direct Chip-on-chip N/A S288c Unstressed log-phase growth (control) YPD medium; mid-log phase N/A
PubMed external link Workman CT et al., Science (New York, N.Y.), 2006 May 19;312(5776):1054-9Workman CT et al., Science (New York, N.Y.), 2006 May 19;312(5776):1054-9 Direct Chip-on-chip N/A S288c N/A N/A N/A
PubMed external link Palavecino MD et al., Journal of amino acids, 2015;2015():484702Palavecino MD et al., Journal of amino acids, 2015;2015():484702 Indirect RT-PCR - wt vs TF mutant Positive S288c Unstressed log-phase growth (control) Control N/A
PubMed external link Ponomarova O et al., Cell systems, 2017 Oct 25;5(4):345-357.e6Ponomarova O et al., Cell systems, 2017 Oct 25;5(4):345-357.e6 Indirect RNA-seq analysis - WT vs TF Deletion Positive S90 Cell cycle/morphology Second acidic phase (day 21): Giant colonies grown on GM solid agar (1% yeast extract, 3% glycerol, 2% agar, 30 mM CaCl2) at room temperature; colonies harvested at day 21, corresponding to the second acidic phase. 1.27164175221559
Indirect RNA-seq analysis - WT vs TF Deletion Positive S90 Unstressed log-phase growth (control) Grown in CDM35 at 30 ºC and harvested at OD600 of 1. Transcription factor gene deletion. 1.27164175221559
PubMed external link Muñoz SA et al., Microbiology (Reading, England), 2020 Jan;166(1):85-92Muñoz SA et al., Microbiology (Reading, England), 2020 Jan;166(1):85-92 Indirect lacZ - wt vs TF mutant Positive SBCY17 Nitrogen source quality/availability minimal medium containing 0.17% Difco yeast nitrogen base (yeast nitrogen base without amino acids and ammonium sulfate) with 2% glucose as a carbon source and 10mM proline as a nitrogen source + 1.3mM leucine for 1h N/A
Indirect lacZ - wt vs TF mutant Positive SBCY17 Nitrogen source quality/availability Cells grown on minimal medium with 2% glucose and 10mM proline up to an OD600nm of 0.5-0.9 were harvested and transferred to fresh medium with 1.3 mM leucine for 1 hour. N/A
Indirect LacZ - WT vs. TF double mutant Positive SBCY20 Nitrogen source quality/availability Cells grown on minimal medium with 2% glucose and 10mM ammonium sulfate up to an OD600nm of 0.5-0.9 were harvested and transferred to fresh medium with 1.3 mM leucine for 1 hour. N/A
Indirect LacZ - WT vs. TF double mutant Positive SBCY20 Unstressed log-phase growth (control) Cells grown on minimal medium with 2% glucose and 10mM ammonium sulfate up to an OD600nm of 0.5-0.9 were harvested and transferred to fresh medium with the same composition for 1 hour. N/A
Indirect LacZ - WT vs. TF double mutant Positive SBCY20 Nitrogen source quality/availability Cells grown on minimal medium with 2% glucose and 10mM proline up to an OD600nm of 0.5-0.9 were harvested and transferred to fresh medium with 1.3 mM leucine for 1 hour. N/A