PubMed
Harbison CT et al., Nature, 2004 Sep 2;431(7004):99-104Harbison CT et al., Nature, 2004 Sep 2;431(7004):99-104
Direct
Chip-on-chip
N/A
S288c
Unstressed log-phase growth (control)
YPD medium; mid-log phase
N/A
PubMed
Fang W et al., Cell reports, 2022 Apr 12;39(2):110672Fang W et al., Cell reports, 2022 Apr 12;39(2):110672
Indirect
RNA-seq analysis - WT vs TF Deletion
Negative
CEN.PK
Carbon source quality/availability
Yeast cells grown to log phase in YPL medium from 0.1 OD. Transcription factor gene deletion.
-0.230366732382966
PubMed
Couvillion M et al., eLife, 2022 May 16;11():Couvillion M et al., eLife, 2022 May 16;11():
Indirect
RNA-seq analysis - WT vs TF Deletion
Negative
BY4741
Unstressed log-phase growth (control)
Overnight cultures grown in YPD medium were diluted to OD600 = 0.05 in 1 L YPD and incubated at 30 °C with 200 rpm shaking to mid-log phase (OD600 = 0.6–0.8). Transcription factor gene deletion.
-0.63927221216997
PubMed
Glazier VE et al., mBio, 2023 Oct 31;14(5):e0152123Glazier VE et al., mBio, 2023 Oct 31;14(5):e0152123
Indirect
RNA-seq analysis - WT vs TF Deletion
Positive
BY4741
Unstressed log-phase growth (control)
Cultured in minimal medium (1.7 g/L YNB, 5 g/L ammonium sulfate, 20 g/L glucose, 80 mg/L histidine, 80 mg/L methionine, 80 mg/L uracil and 240 mg/L leucine) with 200 rpm orbital shaking at 30°C, pH 5.5, until the mid-exponential phase (OD600 = 0.6). Transcription factor gene deletion.
