S.cerevisiae - Yeastract

Regulation Reference(s)

The Yeastract team, is actively curating each regulation evidence code, association type and environmental condition.

Transcription Factor	Target ORF/Genes	References	Evidence Code	Evidence Experiment	Association Type	Strain	Environmental Group	Environmental Condition	Log2FC
Gat1p	YBR208C	PubMed Shamji AF et al., Current biology : CB, 2000 Dec 14-28;10(24):1574-81	Indirect	Microarray analysis - wt vs TF mutant	Positive	S288c	Nitrogen source quality/availability	30 min 50 nM treatment with rapamycin in YPD	N/A
		PubMed Zhao X et al., Scientific reports, 2016 Feb 22;6():21603	Indirect	RT-PCR - wt vs TF mutant	Positive	S288c	Nitrogen source quality/availability	YNB medium, with glutamine or proline	N/A
		PubMed Hong J et al., eLife, 2018 Apr 5;7():Hong J et al., eLife, 2018 Apr 5;7():	Indirect	RNA-seq analysis - WT vs TF Mutant	N/A	FY4	Cell cycle/morphology	Early first acidic phase (day 4): Giant colonies grown on GM solid agar (1% yeast extract, 3% glycerol, 2% agar, 30 mM CaCl2) at room temperature; colonies harvested at day 4, corresponding to the early first acidic phase.	-5.348849199
			Indirect	RNA-seq analysis - WT vs TF Mutant	N/A	FY4	Cell cycle/morphology	Middle first acidic phase (day 7): Giant colonies grown on GM solid agar (1% yeast extract, 3% glycerol, 2% agar, 30 mM CaCl2) at room temperature; colonies harvested at day 7, corresponding to the middle first acidic phase.	-5.070985648
			Indirect	RNA-seq analysis - WT vs TF Mutant	N/A	FY4	Unstressed log-phase growth (control)	Grown in synthetic complete (SC) medium at 30°C to a density of 10^7 cells per mL. Transcription factor gene deletion.	-4.303853201
			Indirect	RNA-seq analysis - WT vs TF Mutant	N/A	FY4	Nitrogen source quality/availability	Cells cultured in ammonium-limited chemostats.Strain bearing single GAT1 missense mutations gat1-1 (W321). Transcription factor gene mutation.	-5.348849199
			Indirect	RNA-seq analysis - WT vs TF Mutant	N/A	FY4	Nitrogen source quality/availability	Cells cultured in ammonium-limited chemostats.Strain bearing single GAT1 missense mutations gat1-2 (C331Y). Transcription factor gene mutation.	-5.070985648
			Indirect	RNA-seq analysis - WT vs TF Mutant	N/A	FY4	Nitrogen source quality/availability	Cells cultured in ammonium-limited chemostats.Strain bearing single GAT1 missense mutations gat1-3 (R345G). Transcription factor gene mutation.	-4.303853201
		PubMed Zhang P et al., Journal of agricultural and food chemistry, 2018 Oct 17;66(41):10881-10889	Indirect	RT-PCR - wt vs TF mutant	Positive	BY4741	Carbon source quality/availability	DELTAgzf3 vs DELTAgzf3+overIndirect of GAT1 gene: strains were first grown in YNB + ammonium sulfate medium, supplemented with 25 mg/L leucine, 25 mg/L histidine, and 25 mg/L methionine overnight at 30.C, then cells were diluted to A600=0.2 and transferred to YPD medium for 48h.	N/A
			Indirect	RT-PCR - wt vs TF mutant	Positive	BY4741	Carbon source quality/availability	wt vs wt-overexpressing truncated Gat1p1-375: Strains were first grown in YNB + ammonium sulfate medium, supplemented with 25 mg/L leucine, 25 mg/L histidine, and 25 mg/L methionine overnight at 30.C, then cells were diluted to A600=0.2 and transferred to YPD medium for 48h.	N/A
			Indirect	RT-PCR - wt vs TF mutant	Positive	BY4741	Carbon source quality/availability	wt vs DELTAgzf3-overexpressing truncated Gat1p1-375: Strains were first grown in YNB + ammonium sulfate medium, supplemented with 25 mg/L leucine, 25 mg/L histidine, and 25 mg/L methionine overnight at 30.C, then cells were diluted to A600=0.2 and transferred to YPD medium for 48h.	N/A
			Indirect	RT-PCR - wt vs TF mutant	Negative	BY4741	Carbon source quality/availability	wt vs DELTAgln3-overexpressing truncated Gat1p1-375: Strains were first grown in YNB + ammonium sulfate medium, supplemented with 25 mg/L leucine, 25 mg/L histidine, and 25 mg/L methionine overnight at 30.C, then cells were diluted to A600=0.2 and transferred to YPD medium for 48h.	N/A
			Indirect	RT-PCR - wt vs TF mutant	Negative	BY4741	Carbon source quality/availability	wt vs DELTAgat1-overexpressing truncated Gat1p1-375: Strains were first grown in YNB + ammonium sulfate medium, supplemented with 25 mg/L leucine, 25 mg/L histidine, and 25 mg/L methionine overnight at 30.C, then cells were diluted to A600=0.2 and transferred to YPD medium for 48h.	N/A
			Indirect	RT-PCR - wt vs TF mutant	Negative	BY4741	Carbon source quality/availability	wt vs DELTAdal80-overexpressing truncated Gat1p1-375: Strains were first grown in YNB + ammonium sulfate medium, supplemented with 25 mg/L leucine, 25 mg/L histidine, and 25 mg/L methionine overnight at 30.C, then cells were diluted to A600=0.2 and transferred to YPD medium for 48h.	N/A