S.cerevisiae - Yeastract

Regulation Reference(s)

The Yeastract team, is actively curating each regulation evidence code, association type and environmental condition.

Transcription Factor	Target ORF/Genes	References	Evidence Code	Evidence Experiment	Association Type	Strain	Environmental Group	Environmental Condition	Log2FC
Gln3p	YBR208C	PubMed Cooper TG et al., Journal of bacteriology, 1990 Feb;172(2):1014-8	Indirect	Northern blot - wt vs TF mutant	Positive	AM100	Nitrogen source quality/availability	Minimal glucose proline medium	N/A
		PubMed Shamji AF et al., Current biology : CB, 2000 Dec 14-28;10(24):1574-81	Indirect	Microarray analysis - wt vs TF mutant	Positive	S288c	Nitrogen source quality/availability	30 min 50 nM treatment with rapamycin in YPD	N/A
		PubMed Staschke KA et al., The Journal of biological chemistry, 2010 May 28;285(22):16893-911	Indirect	Microarray analysis - WT vs TF Deletion	Positive	WY798	Nitrogen source quality/availability	Grown in synthetic medium (SD) (2% dextrose, 0.5% ammonium sulfate) + amino acids w/o histidine (SC medium). Cells treated for 1 h with 200 nM rapamycin. Transcription factor gene deletion.	0.29865832
			Indirect	Microarray analysis - WT vs TF Deletion	Positive	WY798	Unstressed log-phase growth (control)	Grown in synthetic medium (SD) (2% dextrose, 0.5% ammonium sulfate) + amino acids w/o histidine (SC medium). Transcription factor gene deletion.	0.51582007
		PubMed Hernández H et al., Biochemical and biophysical research communications, 2011 Jan 21;404(3):859-64	Direct	ChIP	N/A	S288c	Nitrogen source quality/availability	3-aminotriazole	N/A
		PubMed Zhao X et al., Applied and environmental microbiology, 2014 Jan;80(1):392-8	Indirect	RT-PCR - wt vs TF mutant	Positive	S288c	Nitrogen source quality/availability	Gln3p with S344A S347A S355A mutations or/and Gln3p with truncation of the exact C-terminal NLRS YNB medium supplemented with glutamine (10 mmol/liter). wt S. cerevisiae CEN PK2 strain was used as the control.	N/A
		PubMed Zhang P et al., Journal of agricultural and food chemistry, 2018 Oct 17;66(41):10881-10889	Indirect	RT-PCR - wt vs TF mutant	Positive	BY4741	Carbon source quality/availability	DELTAgzf3 vs DELTAgzf3+overIndirect of GLN3 gene: strains were first grown in YNB + ammonium sulfate medium, supplemented with 25 mg/L leucine, 25 mg/L histidine, and 25 mg/L methionine overnight at 30.C, then cells were diluted to A600=0.2 and transferred to YPD medium for 48h.	N/A
			Indirect	RT-PCR - wt vs TF mutant	Positive	BY4741	Carbon source quality/availability	wt vs wt-overexpressing truncated Gln3p1-653: Strains were first grown in YNB + ammonium sulfate medium, supplemented with 25 mg/L leucine, 25 mg/L histidine, and 25 mg/L methionine overnight at 30.C, then cells were diluted to A600=0.2 and transferred to YPD medium for 48h.	N/A
			Indirect	RT-PCR - wt vs TF mutant	Positive	BY4741	Carbon source quality/availability	wt vs DELTAgzf3-overexpressing truncated Gln3p1-653: Strains were first grown in YNB + ammonium sulfate medium, supplemented with 25 mg/L leucine, 25 mg/L histidine, and 25 mg/L methionine overnight at 30.C, then cells were diluted to A600=0.2 and transferred to YPD medium for 48h.	N/A
			Indirect	RT-PCR - wt vs TF mutant	Negative	BY4741	Carbon source quality/availability	wt vs DELTAgln3-overexpressing truncated Gln3p1-653: Strains were first grown in YNB + ammonium sulfate medium, supplemented with 25 mg/L leucine, 25 mg/L histidine, and 25 mg/L methionine overnight at 30.C, then cells were diluted to A600=0.2 and transferred to YPD medium for 48h.	N/A
			Indirect	RT-PCR - wt vs TF mutant	Negative	BY4741	Carbon source quality/availability	wt vs DELTAgat1-overexpressing truncated Gln3p1-653: Strains were first grown in YNB + ammonium sulfate medium, supplemented with 25 mg/L leucine, 25 mg/L histidine, and 25 mg/L methionine overnight at 30.C, then cells were diluted to A600=0.2 and transferred to YPD medium for 48h.	N/A
			Indirect	RT-PCR - wt vs TF mutant	Negative	BY4741	Carbon source quality/availability	wt vs DELTAdal80-overexpressing truncated Gln3p1-653: Strains were first grown in YNB + ammonium sulfate medium, supplemented with 25 mg/L leucine, 25 mg/L histidine, and 25 mg/L methionine overnight at 30.C, then cells were diluted to A600=0.2 and transferred to YPD medium for 48h.	N/A
		PubMed Rossi MJ et al., Nature, 2021 Apr;592(7853):309-314	Direct	ChIP-exo	N/A	BY4741	Unstressed log-phase growth (control)	YPD medium, 25.C	N/A
		PubMed Chen Y et al., Biochemical and biophysical research communications, 2023 Jun 25;662():31-38	Direct	ChIP	N/A	288c	Unstressed log-phase growth (control)	YNB medium	N/A
		PubMed Hurieva B et al., Nucleic acids research, 2024 Aug 27;52(15):8763-8777	Direct	ChEC-seq - wt vs TF truncations	N/A	BY4741	Unstressed log-phase growth (control)	SD medium	N/A