PubMed
Reimand J et al., Nucleic acids research, 2010 Aug;38(14):4768-77Reimand J et al., Nucleic acids research, 2010 Aug;38(14):4768-77
Indirect
Microarray analysis - WT vs TF Deletion
Positive
BY4741; S288C; R1158
Unstressed log-phase growth (control)
Standard YPD conditions Dataset ewxtracted from The Longhorn Array Database (LAD). Transcription factor gene deletion.
0.60990363237355
PubMed
Matos GS et al., Biochimica et biophysica acta. Molecular and cell biology of lipids, 2021 Sep;1866(9):158983Matos GS et al., Biochimica et biophysica acta. Molecular and cell biology of lipids, 2021 Sep;1866(9):158983
Indirect
RT-PCR - wt vs. TF mutant
Positive
BY4741
Cell cycle/morphology
Cells were cultivated to exponential phase in YPD medium and G1 synchronized with alpha factor. Cells were then released in minimal medium (SD medium) and harvested after 30 or 45 or 60 or 75 min