S.cerevisiae - Yeastract

Regulation Reference(s)

The Yeastract team, is actively curating each regulation evidence code, association type and environmental condition.

Transcription Factor	Target ORF/Genes	References	Evidence Code	Evidence Experiment	Association Type	Strain	Environmental Group	Environmental Condition	Log2FC
Pho4p	YBR296C	PubMed Ogawa N et al., Molecular biology of the cell, 2000 Dec;11(12):4309-21	Indirect	Microarray analysis - wt vs TF mutant	Positive	S288c	Unstressed log-phase growth (control)	Exponential phase cells cultivated in YPD	N/A
		PubMed Zhu H et al., Rapid communications in mass spectrometry : RCM, 2002;16(22):2115-23	Indirect	lacZ - wt vs TF mutant	Positive	S288c	Stress	Alkaline pH stress; Cells cultivated in SC medium at pH 8	N/A
		PubMed Harbison CT et al., Nature, 2004 Sep 2;431(7004):99-104	Direct	Chip-on-chip	N/A	S288c	N/A	N/A	N/A
		PubMed Chua G et al., Proceedings of the National Academy of Sciences of the United States of America, 2006 Aug 8;103(32):12045-50	Indirect	Microarray analysis - wt vs TF overexpression	Positive	N/A	Carbon source quality/availability	Cells grown in selective medium supplemented with 2% raffinose before induction with 2% galactose for 3 h	N/A
		PubMed Nishizawa M et al., PLoS biology, 2008 Dec 23;6(12):2817-30	Direct	Chip-on-chip	N/A	S288c	Ion/metal/phosphate/sulfur/vitamin availability	YNB medium without phosphate + 0,2microM (low Pi; phosphate limitation)	N/A
		PubMed Dos Santos SC et al., Molecular genetics and genomics : MGG, 2011 Dec;286(5-6):333-46	Indirect	lacZ - wt vs TF mutant	Positive	S288c	Stress	quinine stress	N/A
		PubMed Aow JS et al., Nucleic acids research, 2013 May;41(9):4877-87	Direct	ChIP	N/A	S288c	Ion/metal/phosphate/sulfur/vitamin availability	low phosphate	N/A
		PubMed Serra-Cardona A et al., Molecular and cellular biology, 2014 Dec;34(24):4420-35	Indirect	Western blot - wt vs TF mutant	Positive	S288c	Stress	Cells were subjected to pH 8.0 for 90, 120, 180 min followed by immunoblotting using anti-HA antibodies to reveal HA-tagged Pho89	N/A
		PubMed He BZ et al., eLife, 2017 May 9;6():He BZ et al., eLife, 2017 May 9;6():	Indirect	RNA-seq analysis - WT vs TF Deletion	Positive	K699	Unstressed log-phase growth (control)	100 ml of cell culture grown to OD600nm (600 nm) of 1.5.	10.421483793459
			Indirect	RNA-seq analysis - WT vs TF Deletion	Positive	K699	Ion/metal/phosphate/sulfur/vitamin availability	Grown in phosphate-free SC medium (Phosphate starvation) at 30°C with shaking. All transcriptional profiling experiments were done in the pho80∆ background. Transcription factor gene deletion.	10.421483793459
		PubMed Gurvich Y et al., PLoS biology, 2017 Dec;15(12):e2002039	Indirect	RNA-seq - wt vs TF mutant	Positive	BY4741	Ion/metal/phosphate/sulfur/vitamin availability	BY4741; SC medium; Phosphate(i) starvation condition; 30.C; mid-log phase	N/A
			Indirect	RNA-seq - wt vs TF mutant	Positive	BY4741 PHO84-constitutive	Ion/metal/phosphate/sulfur/vitamin availability	BY4741 PHO84c; SC medium; yeast cell recovery into 20mM P(i) from low P(i); 30.C; 2h growth	N/A