S.cerevisiae - Yeastract

Regulation Reference(s)

The Yeastract team, is actively curating each regulation evidence code, association type and environmental condition.

Transcription Factor	Target ORF/Genes	References	Evidence Code	Evidence Experiment	Association Type	Strain	Environmental Group	Environmental Condition	Log2FC
Pho4p	YCR098C	PubMed Almaguer C et al., Eukaryotic cell, 2003 Aug;2(4):729-36	Indirect	Enzyme activity - wt vs TF mutant	Positive	S288c	Unstressed log-phase growth (control)	High Pi - Strains were pregrown in high-Pi, I+ medium were harvested and used to inoculate the following media: high Pi, I?; and high Pi, I+. Following three to five generations of growth, lysates were obtained by glass bead breakage	N/A
		PubMed Onda M et al., Gene, 2004 May 12;332():51-9	Indirect	CAT - wt vs mutant promoter sequence	Positive	S288c	Lipid supplementation	75mM inositol 0,2mM KH2PO4	N/A
		PubMed Harbison CT et al., Nature, 2004 Sep 2;431(7004):99-104	Direct	Chip-on-chip	N/A	S288c	N/A	N/A	N/A
		PubMed Chua G et al., Proceedings of the National Academy of Sciences of the United States of America, 2006 Aug 8;103(32):12045-50	Indirect	Microarray analysis - wt vs TF overexpression	Positive	N/A	Carbon source quality/availability	Cells grown in selective medium supplemented with 2% raffinose before induction with 2% galactose for 3 h	N/A
		PubMed Nishizawa M et al., PLoS biology, 2008 Dec 23;6(12):2817-30	Direct	Chip-on-chip	N/A	S288c	Ion/metal/phosphate/sulfur/vitamin availability	YNB medium without phosphate + 0,2microM (low Pi; phosphate limitation)	N/A
		PubMed He BZ et al., eLife, 2017 May 9;6():He BZ et al., eLife, 2017 May 9;6():	Indirect	RNA-seq analysis - WT vs TF Deletion	Positive	K699	Unstressed log-phase growth (control)	100 ml of cell culture grown to OD600nm (600 nm) of 1.5.	5.53987631493335
			Indirect	RNA-seq analysis - WT vs TF Deletion	Positive	K699	Ion/metal/phosphate/sulfur/vitamin availability	Grown in phosphate-free SC medium (Phosphate starvation) at 30°C with shaking. All transcriptional profiling experiments were done in the pho80∆ background. Transcription factor gene deletion.	5.53987631493335