S.cerevisiae - Yeastract

Regulation Reference(s)

The Yeastract team, is actively curating each regulation evidence code, association type and environmental condition.

Transcription Factor	Target ORF/Genes	References	Evidence Code	Evidence Experiment	Association Type	Strain	Environmental Group	Environmental Condition	Log2FC
Gln3p	YDL170W	PubMed Lee TI et al., Science (New York, N.Y.), 2002 Oct 25;298(5594):799-804	Direct	Chip-on-chip	N/A	S288c	Unstressed log-phase growth (control)	YPD medium; mid-log phase	N/A
		PubMed Harbison CT et al., Nature, 2004 Sep 2;431(7004):99-104	Direct	Chip-on-chip	N/A	S288c	Unstressed log-phase growth (control)	SC medium; mid-log phase	N/A
		PubMed Ishida C et al., Molecular microbiology, 2006 Mar;59(6):1790-806	Indirect	Northern blot - wt vs TF mutant	Positive	S288c	Nitrogen source quality/availability	3-aminotriazole	N/A
		PubMed Workman CT et al., Science (New York, N.Y.), 2006 May 19;312(5776):1054-9	Direct	Chip-on-chip	N/A	S288c	N/A	N/A	N/A
		PubMed Staschke KA et al., The Journal of biological chemistry, 2010 May 28;285(22):16893-911	Indirect	lacZ - wt vs TF mutant	Positive	EG328-1A	Nitrogen source quality/availability	PUGA3-lacZ reporter plasmid was introduced into wt cells or into cells deleted for GCN2 and GLN3. Cells were cultured in synthetic complete medium supplemented with all amino acids except histidine, treated with 3-AT or rapamycin(Rap) for 6h	N/A
			Indirect	lacZ - wt vs TF mutant	Positive	EG328-1A	Nitrogen source quality/availability	PUGA3-lacZ reporter plasmid was introduced into wt cells or into cells deleted for GCN4 and GLN3. Cells were cultured in synthetic complete medium supplemented with all amino acids except histidine, treated with 3-AT or rapamycin(Rap) for 6h	N/A
			Indirect	lacZ - wt vs TF mutant	Positive	EG328-1A	Nitrogen source quality/availability	PUGA3-lacZ reporter plasmid was introduced into wt cells or into cells deleted for GLN3. Cells were cultured in synthetic complete medium supplemented with all amino acids except histidine, treated with rapamycin(Rap) for 6h	N/A
			Indirect	Microarray analysis - WT vs TF Deletion	Negative	WY798	Nitrogen source quality/availability	Grown in synthetic medium (SD) (2% dextrose, 0.5% ammonium sulfate) + amino acids w/o histidine (SC medium). Cells treated for 1 h with 10 mM 3-AT. Transcription factor gene deletion.	-0.23404868
			Indirect	Microarray analysis - WT vs TF Deletion	Negative	WY798	Nitrogen source quality/availability	Grown in synthetic medium (SD) (2% dextrose, 0.5% ammonium sulfate) + amino acids w/o histidine (SC medium). Cells treated for 1 h with 200 nM rapamycin. Transcription factor gene deletion.	-0.1432438
		PubMed Ponomarova O et al., Cell systems, 2017 Oct 25;5(4):345-357.e6	Indirect	RNA-seq analysis - WT vs TF Deletion	Negative	S90	Cell cycle/morphology	Second acidic phase (day 21): Giant colonies grown on GM solid agar (1% yeast extract, 3% glycerol, 2% agar, 30 mM CaCl2) at room temperature; colonies harvested at day 21, corresponding to the second acidic phase.	-1.35924327684851
			Indirect	RNA-seq analysis - WT vs TF Deletion	Negative	S90	Unstressed log-phase growth (control)	Grown in CDM35 at 30 ºC and harvested at OD600 of 1. Transcription factor gene deletion.	-1.35924327684851
		PubMed Rossi MJ et al., Nature, 2021 Apr;592(7853):309-314	Direct	ChIP-exo	N/A	BY4741	Unstressed log-phase growth (control)	YPD medium, 25.C	N/A
		PubMed Hurieva B et al., Nucleic acids research, 2024 Aug 27;52(15):8763-8777	Direct	ChEC-seq - wt vs TF truncations	N/A	BY4741	Unstressed log-phase growth (control)	SD medium	N/A