PubMed
Chua G et al., Proceedings of the National Academy of Sciences of the United States of America, 2006 Aug 8;103(32):12045-50Chua G et al., Proceedings of the National Academy of Sciences of the United States of America, 2006 Aug 8;103(32):12045-50
Indirect
Microarray analysis - wt vs TF overexpression
Positive
N/A
Carbon source quality/availability
Cells grown in selective medium supplemented with 2% raffinose before induction with 2% galactose for 3 h
N/A
PubMed
Alenquer M et al., FEMS yeast research, 2006 Dec;6(8):1130-9Alenquer M et al., FEMS yeast research, 2006 Dec;6(8):1130-9
Indirect
Microarray analysis - WT vs TF Deletion
Negative
W303a
Unstressed log-phase growth (control)
Grown in complete synthetic medium (CSM) when selection was required, or in rich medium (YPD) at 30°C. Temperature shift experiment: exponentially growing yeast cells grown in YPD (A600 = 0.8 − 1.2) at 37°C for 1 h. Transcription factor gene deletion.
-1.02005267
PubMed
Byun JY et al., Microbial cell factories, 2025 Feb 24;24(1):47Byun JY et al., Microbial cell factories, 2025 Feb 24;24(1):47
Indirect
RNA-seq - wt vs TF overexpression
Positive
CEN.PK2-1D
Carbon source quality/availability
YPD medium (36h) (post-diauxic shift)
1.16812929519623
Indirect
RNA-seq analysis - TF Over-expression vs WT
Positive
cen.pk-2-1C
Carbon source quality/availability
RNA extraction at 36h. Medium: YPD (1% yeast extract, 2% peptone, 2% glucose) or synthetic complete (SC) medium supplemented as required for auxotrophic markers. Temperature: 30°C, aerobic shaking. Growth phase: exponential or stationary phase.
