S.cerevisiae - Yeastract

Regulation Reference(s)

The Yeastract team, is actively curating each regulation evidence code, association type and environmental condition.

Transcription Factor	Target ORF/Genes	References	Evidence Code	Evidence Experiment	Association Type	Strain	Environmental Group	Environmental Condition	Log2FC
Gat1p	YDL210W	PubMed Steber CM et al., Proceedings of the National Academy of Sciences of the United States of America, 1995 Dec 19;92(26):12490-4	Indirect	Northern blot - wt vs TF mutant	Positive	TCY1	Nitrogen source quality/availability	Wickerham's minimal medium + proline	N/A
		PubMed Shamji AF et al., Current biology : CB, 2000 Dec 14-28;10(24):1574-81	Indirect	Microarray analysis - wt vs TF mutant	Positive	S288c	Nitrogen source quality/availability	30 min 50 nM treatment with rapamycin in YPD	N/A
		PubMed Luzzani C et al., Microbiology (Reading, England), 2007 Nov;153(Pt 11):3677-3684	Indirect	lacZ - wt vs TF mutant	Negative	SIGMA1278b	Nitrogen source quality/availability	wt versus gat1DELTA minimal media with proline as nitrogen source and glucose	N/A
			Indirect	lacZ - wt vs TF mutant	Positive	SIGMA1278b	Nitrogen source quality/availability	wt versus gat1DELTA minimal media with proline as nitrogen source and glucose, supplemented with 0.1mM GABA	N/A
		PubMed Georis I et al., Molecular and cellular biology, 2009 Jul;29(13):3803-15	Direct	ChIP	N/A	S288c	Nitrogen source quality/availability	Exponential-phase cells cultivated in synthetic growth medium containing 0.1% proline as nitrogen source	N/A
		PubMed Zhao X et al., Scientific reports, 2016 Feb 22;6():21603	Indirect	RT-PCR - wt vs TF mutant	Positive	S288c	Nitrogen source quality/availability	YNB medium, with glutamine or proline	N/A
		PubMed Palavecino-Ruiz M et al., Microbiology (Reading, England), 2017 Nov;163(11):1692-1701	Indirect	lacZ - wt vs TF mutant	Positive	23344c	Nitrogen source quality/availability	Minimal medium (0.17% Difco yeast nitrogen base, 2% glucose and 10mM proline) after incubation with 0.1mM GABA during 1h .	N/A
			Indirect	lacZ - wt vs TF mutant	Negative	23344c	Nitrogen source quality/availability	wt and DELTAgln3DELTAgat1 cells carrying the Yep-357-UGA4-lacZ plasmid were grown on 10mM proline	N/A
			Indirect	lacZ - wt vs TF mutant	Positive	23344c	Nitrogen source quality/availability	wt and DELTAgln3DELTAgat1DELTAleu3 cells carrying the Yep-357-UGA4-lacZ plasmid were grown on 10mM proline and incubated with 0.1mM GABA for 60 min	N/A
			Indirect	lacZ - wt vs TF mutant	Positive	23344c	Nitrogen source quality/availability	wt and DELTAgat1 cells carrying the Yep-357-UGA4-lacZ plasmid were grown on 10mM proline and incubated with 0.1mM GABA for 60 min	N/A
			Indirect	lacZ - wt vs TF mutant	Positive	23344c	Nitrogen source quality/availability	wt and DELTAgat1DELTAleu3 cells carrying the Yep-357-UGA4-lacZ plasmid were grown on 10mM proline and incubated with 0.1mM GABA for 60 min	N/A
		PubMed Hong J et al., eLife, 2018 Apr 5;7():Hong J et al., eLife, 2018 Apr 5;7():	Indirect	RNA-seq analysis - WT vs TF Mutant	N/A	FY4	Cell cycle/morphology	Middle first acidic phase (day 7): Giant colonies grown on GM solid agar (1% yeast extract, 3% glycerol, 2% agar, 30 mM CaCl2) at room temperature; colonies harvested at day 7, corresponding to the middle first acidic phase.	-1.842535164
			Indirect	RNA-seq analysis - WT vs TF Mutant	N/A	FY4	Unstressed log-phase growth (control)	Grown in synthetic complete (SC) medium at 30°C to a density of 10^7 cells per mL. Transcription factor gene deletion.	-2.540606791
			Indirect	RNA-seq analysis - WT vs TF Mutant	N/A	FY4	Nitrogen source quality/availability	Cells cultured in ammonium-limited chemostats.Strain bearing single GAT1 missense mutations gat1-2 (C331Y). Transcription factor gene mutation.	-1.842535164
			Indirect	RNA-seq analysis - WT vs TF Mutant	N/A	FY4	Nitrogen source quality/availability	Cells cultured in ammonium-limited chemostats.Strain bearing single GAT1 missense mutations gat1-3 (R345G). Transcription factor gene mutation.	-2.540606791