S.cerevisiae - Yeastract

Regulation Reference(s)

The Yeastract team, is actively curating each regulation evidence code, association type and environmental condition.

Transcription Factor	Target ORF/Genes	References	Evidence Code	Evidence Experiment	Association Type	Strain	Environmental Group	Environmental Condition	Log2FC
Ixr1p	YDR044W	PubMed Castro-Prego R et al., FEMS yeast research, 2010 May;10(3):309-21	Direct	EMSA	N/A	BY4741	In vitro	Ixr1p binds to sequences extending from -534 to -509, -526 to -516, and from -497 to -450 (P497-450) of the HEM13 promoter, at 0. and room temperature	N/A
			Direct	ChIP	N/A	Z1580	Oxygen availability	Cells grown under aerobic or hypoxic conditions	N/A
			Indirect	Northern blot - wt vs TF mutant	Negative	BY4741 and W303	Unstressed log-phase growth (control)	wt vs DELTAixr1: under aerobic conditions	N/A
			Indirect	Northern blot - wt vs TF mutant	Positive	BY4741 and W303	Oxygen availability	wt vs DELTAixr1: under hypoxic conditions	N/A
			Indirect	alpha-galactosidase - wt vs TF mutant	Negative	BY4741	Oxygen availability	wt vs DELTAixr1 cells expressing the HEM13 promoter region P421-179 fused to the reporter gene MEL1 under hypoxic conditions	N/A
			Indirect	alpha-galactosidase - wt vs TF mutant	Positive	BY4741	Oxygen availability	wt vs DELTAixr1 cells expressing the HEM13 promoter region P577-419 fused to the reporter gene MEL1 under hypoxic conditions	N/A
		PubMed Liu J et al., Antioxidants & redox signaling, 2013 Dec 1;19(16):1916-27	Indirect	RT-PCR - wt vs TF mutant	Positive	S288c	Unstressed log-phase growth (control)	exponential phase	N/A
		PubMed Vizoso-Vázquez A et al., Biochimica et biophysica acta. Gene regulatory mechanisms, 2017 Feb;1860(2):256-269	Indirect	RT-PCR - wt vs TF mutant	Positive	W303	Oxygen availability	Hypoxic conditions	N/A
		PubMed Krakowiak J et al., eLife, 2018 Feb 2;7():	Indirect	Microarray analysis - WT vs TF Deletion	Negative	W303a	Unstressed log-phase growth (control)	Grown overnight in complete SD medium with specific amino acid supplements and 2% glucose, then diluted to OD600 0.4 in fresh medium and cultured at 30 °C with shaking until mid-log phase (OD600 0.6). Transcription factor gene deletion.	-0.33182667
		PubMed Krakowiak J et al., eLife, 2018 Feb 2;7():	Indirect	Microarray analysis - WT vs TF Deletion	Negative	W303a	Stress	Grown overnight in complete SD medium with specific amino acid supplements and 2% glucose, then diluted to OD600 0.4 in fresh medium and cultured at 30 °C with shaking until mid-log phase (OD600 0.6). Cisplatin treatment (600 μM in DMSO), incubated for 4 hours at 30 °C in the dark with agitation. Transcription factor gene deletion.	-0.34549333