PubMed
Onda M et al., Gene, 2004;():Onda M et al., Gene, 2004;():
Indirect
Microarray analysis - WT vs TF mutant
Positive
S288c
YPGal + Adenine
PubMed
Boer VM et al., FEMS Yeast Res, 2005 Jul;5(10):885-97Boer VM et al., FEMS Yeast Res, 2005 Jul;5(10):885-97
Indirect
Microarray analysis - WT vs TF mutant
Negative
CEN.PK540-3A
Ammonium-limitation: Defined synthetic medium (NH4)2SO4, 1.0 g/l;K2SO4, 5.3 g/l;KH2PO4, 3.0 g/l; MgSO4.7H2O, 0.5 g/l; vitamins and trace elements.
PubMed
Tang L et al., BMC Genomics, 2006;():Tang L et al., BMC Genomics, 2006;():
Indirect
Microarray analysis - WT vs TF overexpression
Positive
BY4741
The cells were cultured in uracil dropout medium (YNB-AA (Sigma) 6.7 g/L, 0.77 g/L, Ura DO Supp., and 2% glucose supplemented with G418 at 200 mg/L) with shaking at 30.C. The cells were harvested in log-phase growth (A600 ~ 0.7).
PubMed
Holland P et al., Nucleic Acids Res, 2019 Jun 4;47(10):4986-5000Holland P et al., Nucleic Acids Res, 2019 Jun 4;47(10):4986-5000
Direct
ChIP-exo
N/A
CEN.PK 113-5D
Cells were cultivated in chemostats in respiratory glucose limited medium with a dilution rate of 0.1 h-1 at 30.C. Samples were collected after steady state was achieved for 48-60 h
