S.cerevisiae - Yeastract

Regulation Reference(s)

The Yeastract team, is actively curating each regulation evidence code, association type and environmental condition.

Transcription Factor	Target ORF/Genes	References	Evidence Code	Evidence Experiment	Association Type	Strain	Environmental Group	Environmental Condition	Log2FC
Swi4p	YDR297W	PubMed Iyer VR et al., Nature, 2001 Jan 25;409(6819):533-8	Direct	Chip-on-chip	N/A	S288c	N/A	N/A	N/A
		PubMed Chua G et al., Proceedings of the National Academy of Sciences of the United States of America, 2006 Aug 8;103(32):12045-50	Indirect	Microarray analysis - wt vs TF overexpression	Positive	N/A	Carbon source quality/availability	Cells grown in selective medium supplemented with 2% raffinose before induction with 2% galactose for 3 h	N/A
		PubMed Hendler A et al., PLoS genetics, 2017 May;13(5):e1006778	Indirect	RNA-seq analysis - WT vs TF Deletion	Negative	15Daub	Unstressed log-phase growth (control)	Yeast strains were grown in rich medium at 30°C to an optical density at 600 nm (OD600) of 0.5.	-0.738810425200739
			Indirect	RNA-seq analysis - WT vs TF Deletion	Positive	15Daub	Unstressed log-phase growth (control)	Yeast growth media (yeast-peptone-dextrose [YPD], yeast-peptone-acetate, and synthetic complete) were of standard composition	0.361262747652352
			Indirect	RNA-seq analysis - WT vs TF Deletion	Negative	15Daub	Cell cycle/morphology	Cells were grown in 7 ml of SC over-night at 30°C. Cells were synchronized by alpha factor arrest and release and immediately collected. Transcription factor gene deletion.	-0.738810425200739
			Indirect	RNA-seq analysis - WT vs TF Deletion	Positive	15Daub	Cell cycle/morphology	Cells were grown in 7 ml of SC over-night at 30°C. Cells were synchronized by alpha factor arrest and release and after 30 minutes they were collected. Transcription factor gene deletion.	0.361262747652352
		PubMed Matos GS et al., Biochimica et biophysica acta. Molecular and cell biology of lipids, 2021 Sep;1866(9):158983	Indirect	RT-PCR - wt vs. TF mutant	Positive	BY4741	Cell cycle/morphology	Cells were cultivated to exponential phase in YPD medium and G1 synchronized with alpha factor. Cells were then released in minimal medium (SD medium) and harvested after 30 min	N/A