S.cerevisiae - Yeastract

Regulation Reference(s)

The Yeastract team, is actively curating each regulation evidence code, association type and environmental condition.

Transcription Factor	Target ORF/Genes	References	Evidence Code	Evidence Experiment	Association Type	Strain	Environmental Group	Environmental Condition	Log2FC
Gat1p	YDR421W	PubMed Lee K et al., Molecular microbiology, 2013 Jun;88(6):1120-34	Indirect	RT-PCR - wt vs TF mutant	Positive	S288c	Stress	BY4741 gat1DELTA and gln3DELTAgat1DELTA cells cultured in YPD medium until early exponential phase and then treated with 0.2 microg mlâˆ’1 rapamycin (Rap) for 1 h.	N/A
		PubMed Chen X et al., Journal of biotechnology, 2017 Jan 20;242():83-91	Indirect	RT-PCR - wt vs TF mutant	Positive	S288c	Unstressed log-phase growth (control)	YS58 strain with plasmid containing GAT1 ORF of MT2 or YS58 strains cloned into SalI and XbaI of ADH1 promoter and terminator cloned into Escherichia coli-Yeast shuttle vector - Overexpression of GLN3; cells grown in synthetic complete medium	N/A
			Indirect	RT-PCR - wt vs TF mutant	Positive	YS58	Nitrogen source quality/availability	wt and GAT1 (gene from MT2 strain) over-expressing strains were grown for 12, 24, 48 and 72 hours in medium used for fermentation containing 30 g/L glucose, 0.5 g/L MgSO4Â·7H2O, 0.5 g/L KH2PO4, and 5 g/L L-phenylalanine (l-Phe), as a sole nitrogen source.	N/A
			Indirect	RT-PCR - wt vs TF mutant	Positive	YS58	Nitrogen source quality/availability	wt and GAT1 (gene from YS58 strain) over-expressing strains were grown for 12, 24, 48 and 72 hours in medium used for fermentation containing 30 g/L glucose, 0.5 g/L MgSO4Â·7H2O, 0.5 g/L KH2PO4, and 5 g/L L-phenylalanine (l-Phe), as a sole nitrogen source.	N/A
		PubMed Hong J et al., eLife, 2018 Apr 5;7():Hong J et al., eLife, 2018 Apr 5;7():	Indirect	RNA-seq analysis - WT vs TF Mutant	N/A	FY4	Cell cycle/morphology	Early first acidic phase (day 4): Giant colonies grown on GM solid agar (1% yeast extract, 3% glycerol, 2% agar, 30 mM CaCl2) at room temperature; colonies harvested at day 4, corresponding to the early first acidic phase.	-0.765248209
			Indirect	RNA-seq analysis - WT vs TF Mutant	N/A	FY4	Nitrogen source quality/availability	Cells cultured in ammonium-limited chemostats.Strain bearing single GAT1 missense mutations gat1-1 (W321). Transcription factor gene mutation.	-0.765248209
		PubMed Wang YP et al., BioMed research international, 2020;2020():6802512	Indirect	RT-PCR - wt vs. TF mutant	Negative	alfa-5	Unstressed log-phase growth (control)	YPD medium	N/A