Yeastract
YEASTRACT+ INESC-ID IST IBB
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Regulation Reference(s)

The Yeastract team, is actively curating each regulation evidence code, association type and environmental condition.
Transcription
Factor		 Target
ORF/Genes		 References Evidence
Code		 Evidence
Experiment		 Association
Type		 Strain Environmental
Group		 Environmental
Condition		 Log2FC
Kar4p YDR446W PubMed external link Lahav R et al., Molecular and cellular biology, 2007 Feb;27(3):818-29Lahav R et al., Molecular and cellular biology, 2007 Feb;27(3):818-29 Indirect Microarray analysis - wt vs TF mutant Positive S288c Unstressed log-phase growth (control) Cells growing in YEPD growth medium treated with methanol for 90 minutes (control) N/A
PubMed external link Ensinck I et al., eLife, 2023 Jul 25;12():Ensinck I et al., eLife, 2023 Jul 25;12(): Indirect RNA-seq analysis - WT vs TF Deletion Positive FW1511 Cell cycle/morphology 0 h: RNA-seq performed under sporulation conditions in SK1 background. Overnight YPD cultures diluted to OD600 0.5 in pre-sporulation medium (SPS: 0.5% yeast extract, 1% peptone, 0.17% YNB, 1% potassium acetate, 0.5% ammonium sulfate, 1% glucose) for 16-18 h at 30°C. Washed and transferred to 1% potassium acetate (SPO medium) at OD600 1.9. Samples collected at 0 h post-transfer to SPO. 1.570853
Indirect RNA-seq analysis - WT vs TF Deletion Positive FW1511 Cell cycle/morphology 2 h: RNA-seq performed under sporulation conditions in SK1 background. Overnight YPD cultures diluted to OD600 0.5 in pre-sporulation medium (SPS: 0.5% yeast extract, 1% peptone, 0.17% YNB, 1% potassium acetate, 0.5% ammonium sulfate, 1% glucose) for 16-18 h at 30°C. Washed and transferred to 1% potassium acetate (SPO medium) at OD600 1.9. Samples collected at 2 h post-transfer to SPO. 0.512685
Indirect RNA-seq analysis - WT vs TF Deletion Positive FW1511 Cell cycle/morphology 4 h: RNA-seq performed under sporulation conditions in SK1 background. Overnight YPD cultures diluted to OD600 0.5 in pre-sporulation medium (SPS: 0.5% yeast extract, 1% peptone, 0.17% YNB, 1% potassium acetate, 0.5% ammonium sulfate, 1% glucose) for 16-18 h at 30°C. Washed and transferred to 1% potassium acetate (SPO medium) at OD600 1.9. Samples collected at 4 h post-transfer to SPO. 1.143978
Indirect RNA-seq analysis - WT vs TF Deletion Positive FW1511 Cell cycle/morphology 6 h: RNA-seq performed under sporulation conditions in SK1 background. Overnight YPD cultures diluted to OD600 0.5 in pre-sporulation medium (SPS: 0.5% yeast extract, 1% peptone, 0.17% YNB, 1% potassium acetate, 0.5% ammonium sulfate, 1% glucose) for 16-18 h at 30°C. Washed and transferred to 1% potassium acetate (SPO medium) at OD600 1.9. Samples collected at 6 h post-transfer to SPO. 0.494975
Indirect RNA-seq analysis - WT vs TF Deletion Negative FW1511 Cell cycle/morphology 8 h: RNA-seq performed under sporulation conditions in SK1 background. Overnight YPD cultures diluted to OD600 0.5 in pre-sporulation medium (SPS: 0.5% yeast extract, 1% peptone, 0.17% YNB, 1% potassium acetate, 0.5% ammonium sulfate, 1% glucose) for 16-18 h at 30°C. Washed and transferred to 1% potassium acetate (SPO medium) at OD600 1.9. Samples collected at 8 h post-transfer to SPO. -1.045548