S.cerevisiae - Yeastract

Regulation Reference(s)

The Yeastract team, is actively curating each regulation evidence code, association type and environmental condition.

Transcription Factor	Target ORF/Genes	References	Evidence Code	Evidence Experiment	Association Type	Strain	Environmental Group	Environmental Condition	Log2FC
Gln3p	YEL009C	PubMed Lee TI et al., Science (New York, N.Y.), 2002 Oct 25;298(5594):799-804	Direct	Chip-on-chip	N/A	S288c	Unstressed log-phase growth (control)	YPD medium; mid-log phase	N/A
		PubMed Harbison CT et al., Nature, 2004 Sep 2;431(7004):99-104	Direct	Chip-on-chip	N/A	S288c	Unstressed log-phase growth (control)	YPD medium; mid-log phase	N/A
		PubMed Staschke KA et al., The Journal of biological chemistry, 2010 May 28;285(22):16893-911	Indirect	lacZ - wt vs TF mutant	Positive	EG328-1A	Nitrogen source quality/availability	GCN4-lacZ reporter plasmid (GCN4-uORF-lacZ) was introduced into wt cells vs cells deleted for GLN3andGCN2. Cells were treated with 3-AT	N/A
			Indirect	lacZ - wt vs TF mutant	Positive	EG328-1A	Carbon source quality/availability	GCN4-lacZ reporter plasmid (GCN4-uORF-lacZ) was introduced into wt cells vs cells deleted for GLN3 or GLN3andGCN2. Cells were treated with rapamicin	N/A
			Indirect	Microarray analysis - WT vs TF Deletion	Positive	WY798	Nitrogen source quality/availability	Grown in synthetic medium (SD) (2% dextrose, 0.5% ammonium sulfate) + amino acids w/o histidine (SC medium). Cells treated for 1 h with 200 nM rapamycin. Transcription factor gene deletion.	0.11103131
		PubMed Reimand J et al., Nucleic acids research, 2010 Aug;38(14):4768-77	Indirect	Microarray analysis - WT vs TF Deletion	Positive	BY4741; S288C; R1158	Unstressed log-phase growth (control)	Standard YPD conditions Dataset ewxtracted from The Longhorn Array Database (LAD). Transcription factor gene deletion.	0.31918969639298
		PubMed Ponomarova O et al., Cell systems, 2017 Oct 25;5(4):345-357.e6	Indirect	RNA-seq analysis - WT vs TF Deletion	Negative	S90	Cell cycle/morphology	Second acidic phase (day 21): Giant colonies grown on GM solid agar (1% yeast extract, 3% glycerol, 2% agar, 30 mM CaCl2) at room temperature; colonies harvested at day 21, corresponding to the second acidic phase.	-0.583043421244833
			Indirect	RNA-seq analysis - WT vs TF Deletion	Negative	S90	Unstressed log-phase growth (control)	Grown in CDM35 at 30 ºC and harvested at OD600 of 1. Transcription factor gene deletion.	-0.583043421244833
		PubMed Rossi MJ et al., Nature, 2021 Apr;592(7853):309-314	Direct	ChIP-exo	N/A	BY4741	Unstressed log-phase growth (control)	YPD medium, 25.C	N/A
		PubMed Hurieva B et al., Nucleic acids research, 2024 Aug 27;52(15):8763-8777	Direct	ChEC-seq - wt vs TF truncations	N/A	BY4741	Unstressed log-phase growth (control)	SD medium	N/A