PubMed
Staschke KA et al., The Journal of biological chemistry, 2010 May 28;285(22):16893-911Staschke KA et al., The Journal of biological chemistry, 2010 May 28;285(22):16893-911
Indirect
Microarray analysis - WT vs TF Deletion
Positive
WY798
Nitrogen source quality/availability
Grown in synthetic medium (SD) (2% dextrose, 0.5% ammonium sulfate) + amino acids w/o histidine (SC medium). Cells treated for 1 h with 200 nM rapamycin. Transcription factor gene deletion.
0.11103131
PubMed
Sato A et al., Bioscience, biotechnology, and biochemistry, 2021 Feb 24;85(3):587-599Sato A et al., Bioscience, biotechnology, and biochemistry, 2021 Feb 24;85(3):587-599
Indirect
RT-PCR - wt vs. TF double mutant
Positive
X2180-1B
Nitrogen source quality/availability
Nitrogen starved cells (Cells were grown in YPD medium to an early logarithmic phase. For nitrogen starvation, cells were washed once and further cultured for 6 h in SD-N ((0.17% yeast nitrogen base without amino acids and ammonium sulfate, and 2% glucose)).