PubMed
Le Crom S et al., Molecular and cellular biology, 2002 Apr;22(8):2642-9Le Crom S et al., Molecular and cellular biology, 2002 Apr;22(8):2642-9
Indirect
Microarray analysis - TF GOF vs WT
Positive
SEY6210
Unstressed log-phase growth (control)
100 ml of cell culture grown to OD600nm (600 nm) of 1.5.
0.18293642
PubMed
Wang X et al., Frontiers in microbiology, 2017;8():367Wang X et al., Frontiers in microbiology, 2017;8():367
Indirect
RNA-seq analysis - WT vs TF Deletion
Negative
BY4741
Unstressed log-phase growth (control)
Cells harvested from 300 ml of culture (specific medium not clear) at an OD600 of 0.4. Transcription factor gene overexpression pAFT2-1up vs aft2Δ strain.
-0.503048090698537
Indirect
RNA-seq analysis - WT vs TF Deletion
Negative
BY4741
Stress
Grown in SD medium until log phase (OD600 ≈ 1.6-2.0) at 30 ºC, in the presence of 5 mM vanillin (inhibitor). Transcription factor gene deletion.