S.cerevisiae - Yeastract

Regulation Reference(s)

The Yeastract team, is actively curating each regulation evidence code, association type and environmental condition.

Transcription Factor	Target ORF/Genes	References	Evidence Code	Evidence Experiment	Association Type	Strain	Environmental Group	Environmental Condition	Log2FC
Rph1p	YER142C	PubMed Reimand J et al., Nucleic acids research, 2010 Aug;38(14):4768-77	Indirect	Microarray analysis - WT vs TF Deletion	Negative	BY4741; S288C; R1158	Unstressed log-phase growth (control)	Standard YPD conditions Dataset ewxtracted from The Longhorn Array Database (LAD). Transcription factor gene deletion.	-0.31825440143454
		PubMed Li F et al., Nucleic acids research, 2017 May 19;45(9):5183-5197	Indirect	RT-PCR - wt vs TF overexpression	Negative	BY4741	Stress	YPD + methyl-methane sulphonate (MMS)	N/A
		PubMed Shu WJ et al., Nucleic acids research, 2020 Sep 4;48(15):8360-8373	Indirect	RNA-seq analysis - WT vs TF Deletion	Negative	BY4741	Carbon source quality/availability	Steady state carbon-limited chemostat cultures operated at a dilution rate of 0.05 per h by continuous addition of medium (feed concentration of glucose, 10 g/liter) at 30°C. pH controlled at 5, aerated with 0.5 volume of air/volume of liquid/min and with 800 rpm agitation. Transcription factor gene deletion.	-0.976960038566516
			Indirect	RNA-seq analysis - WT vs TF Deletion	Positive	BY4741	Carbon source quality/availability	Ethanol medium - Overexpression media lacked leucine to ensure plasmid selection. The cultures grown for 70 h in ethanol medium were diluted twice with a fresh medium to ensure continuous growth.	0.303009114924827
			Indirect	RNA-seq analysis - WT vs TF Deletion	Negative	BY4741	Unstressed log-phase growth (control)	Grown in YPD to OD600 of 0.8 at 30 ºC. Transcription factor gene deletion.	-0.976960038566516
			Indirect	RNA-seq analysis - WT vs TF Deletion	Positive	BY4741	Nitrogen source quality/availability	Grown in YPD to OD600 of 0.8 at 30 ºC. Treated with rapamycin for 2h (100 nM). Transcription factor gene deletion.	0.303009114924827
		PubMed Glazier VE et al., mBio, 2023 Oct 31;14(5):e0152123	Indirect	RNA-seq analysis - WT vs TF Deletion	Negative	BY4741	Unstressed log-phase growth (control)	Cultured in minimal medium (1.7 g/L YNB, 5 g/L ammonium sulfate, 20 g/L glucose, 80 mg/L histidine, 80 mg/L methionine, 80 mg/L uracil and 240 mg/L leucine) with 200 rpm orbital shaking at 30°C, pH 5.5, until the mid-exponential phase (OD600 = 0.6). Transcription factor gene deletion.	-0.474021285944531