Yeastract
YEASTRACT+ INESC-ID IST IBB
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Regulation Reference(s)

The Yeastract team, is actively curating each regulation evidence code, association type and environmental condition.
Transcription
Factor		 Target
ORF/Genes		 References Evidence
Code		 Evidence
Experiment		 Association
Type		 Strain Environmental
Group		 Environmental
Condition		 Log2FC
Rph1p YER169W PubMed external link Cipollina C et al., Microbiology (Reading, England), 2008 Jan;154(Pt 1):337-346Cipollina C et al., Microbiology (Reading, England), 2008 Jan;154(Pt 1):337-346 Indirect Microarray analysis - WT vs TF Deletion Positive SLY34 Unstressed log-phase growth (control) Grown in rich medium (YPD) at 30 °C to log phase. Transcription factor gene deletion. 5.60102
PubMed external link Schroeder EA et al., Cell metabolism, 2013 Jun 4;17(6):954-964Schroeder EA et al., Cell metabolism, 2013 Jun 4;17(6):954-964 Indirect Microarray analysis - WT vs TF Deletion Negative DBY2006 Stress 24h men: Cultures grown in minimal dextrose medium supplemented with essential amino acids at 30°C, 200 rpm. Cells treated with 50 µM menadione during exponential growth (OD600 0.5 to 2.0). RNA harvested at 24 h (day 1 of chronological lifespan, post-diauxic/stationary phase). -2.516965
Indirect Microarray analysis - WT vs TF Deletion Negative DBY2006 Stress 2h men: Cultures grown in minimal dextrose medium supplemented with essential amino acids at 30°C, 200 rpm. Cells treated with 50 µM menadione during exponential growth (OD600 0.5 to 2.0). RNA harvested at 2 h (exponential phase). -2.662077
Indirect Microarray analysis - WT vs TF Deletion Negative DBY2006 Carbon source quality/availability 24h nt: Cultures grown in minimal dextrose medium supplemented with essential amino acids at 30°C, 200 rpm (OD600 0.5 to 2.0). RNA harvested at 24 h (day 1 of chronological lifespan, post-diauxic/stationary phase). -2.643894
Indirect Microarray analysis - WT vs TF Deletion Negative DBY2006 Unstressed log-phase growth (control) 2h nt: Cultures grown in minimal dextrose medium supplemented with essential amino acids at 30°C, 200 rpm (OD600 0.5 to 2.0). RNA harvested at 2 h (exponential phase). -2.73161
PubMed external link Wang J et al., Scientific reports, 2017 Feb 2;7():41850Wang J et al., Scientific reports, 2017 Feb 2;7():41850 Indirect Western blot - wt vs TF mutant Positive JKM179 Stress YPD + methyl-methane sulphonate (MMS) N/A
PubMed external link Shu WJ et al., Nucleic acids research, 2020 Sep 4;48(15):8360-8373Shu WJ et al., Nucleic acids research, 2020 Sep 4;48(15):8360-8373 Indirect RNA-seq analysis - WT vs TF Deletion Positive BY4741 Carbon source quality/availability Steady state carbon-limited chemostat cultures operated at a dilution rate of 0.05 per h by continuous addition of medium (feed concentration of glucose, 10 g/liter) at 30°C. pH controlled at 5, aerated with 0.5 volume of air/volume of liquid/min and with 800 rpm agitation. Transcription factor gene deletion. 8.87651958952335
Indirect RNA-seq analysis - WT vs TF Deletion Positive BY4741 Carbon source quality/availability Ethanol medium - Overexpression media lacked leucine to ensure plasmid selection. The cultures grown for 70 h in ethanol medium were diluted twice with a fresh medium to ensure continuous growth. 7.95203774531986
Indirect RNA-seq analysis - WT vs TF Deletion Positive BY4741 Unstressed log-phase growth (control) Grown in YPD to OD600 of 0.8 at 30 ºC. Transcription factor gene deletion. 8.87651958952335
Indirect RNA-seq analysis - WT vs TF Deletion Positive BY4741 Nitrogen source quality/availability Grown in YPD to OD600 of 0.8 at 30 ºC. Treated with rapamycin for 2h (100 nM). Transcription factor gene deletion. 7.95203774531986
PubMed external link Fang W et al., Cell reports, 2022 Apr 12;39(2):110672Fang W et al., Cell reports, 2022 Apr 12;39(2):110672 Indirect RNA-seq analysis - WT vs TF Deletion Positive CEN.PK Carbon source quality/availability Yeast cells grown to log phase in YPL medium from 0.1 OD. Transcription factor gene deletion. 8.93784823984232
Indirect RNA-seq analysis - WT vs TF Deletion Positive CEN.PK Carbon source quality/availability Yeast cells grown to log phase in YPL medium from 0.1 OD with switch to SL minimal medium for 1h. Transcription factor gene deletion. 8.26995633130982
Indirect RNA-seq analysis - WT vs TF Deletion Positive CEN.PK Carbon source quality/availability Yeast cells grown to log phase in YPL medium from 0.1 OD with switch to SL minimal medium for 3h. Transcription factor gene deletion. 9.48668794752401
PubMed external link Couvillion M et al., eLife, 2022 May 16;11():Couvillion M et al., eLife, 2022 May 16;11(): Indirect RNA-seq analysis - WT vs TF Deletion Positive BY4741 Unstressed log-phase growth (control) Overnight cultures grown in YPD medium were diluted to OD600 = 0.05 in 1 L YPD and incubated at 30 °C with 200 rpm shaking to mid-log phase (OD600 = 0.6–0.8). Transcription factor gene deletion. 7.32705605701467
PubMed external link Glazier VE et al., mBio, 2023 Oct 31;14(5):e0152123Glazier VE et al., mBio, 2023 Oct 31;14(5):e0152123 Indirect RNA-seq analysis - WT vs TF Deletion Positive BY4741 Unstressed log-phase growth (control) Cultured in minimal medium (1.7 g/L YNB, 5 g/L ammonium sulfate, 20 g/L glucose, 80 mg/L histidine, 80 mg/L methionine, 80 mg/L uracil and 240 mg/L leucine) with 200 rpm orbital shaking at 30°C, pH 5.5, until the mid-exponential phase (OD600 = 0.6). Transcription factor gene deletion. 8.79138194289622