S.cerevisiae - Yeastract

Regulation Reference(s)

The Yeastract team, is actively curating each regulation evidence code, association type and environmental condition.

Transcription Factor	Target ORF/Genes	References	Evidence Code	Evidence Experiment	Association Type	Strain	Environmental Group	Environmental Condition	Log2FC
Gat1p	YFL021W	PubMed Coffman JA et al., Journal of bacteriology, 1997 Jun;179(11):3416-29	Indirect	lacZ - wt vs TF mutant	Negative	TCY1	Nitrogen source quality/availability	Minimal medium + proline	N/A
		PubMed Georis I et al., Molecular and cellular biology, 2009 Jul;29(13):3803-15	Direct	ChIP	N/A	S288c	Nitrogen source quality/availability	glutamine/proline	N/A
		PubMed Reimand J et al., Nucleic acids research, 2010 Aug;38(14):4768-77	Indirect	Microarray analysis - WT vs TF Deletion	Positive	BY4741; S288C; R1158	Unstressed log-phase growth (control)	Standard YPD conditions Dataset ewxtracted from The Longhorn Array Database (LAD). Transcription factor gene deletion.	1.7877282094361
		PubMed Chen X et al., Journal of biotechnology, 2017 Jan 20;242():83-91	Indirect	RT-PCR - wt vs TF mutant	Positive	S288c	Unstressed log-phase growth (control)	YS58 strain with plasmid containing GAT1 ORF of MT2 or YS58 strains cloned into SalI and XbaI of ADH1 promoter and terminator cloned into Escherichia coli-Yeast shuttle vector - Overexpression of GLN3; cells grown in synthetic complete medium	N/A
			Indirect	RT-PCR - wt vs TF mutant	Positive	YS58	Nitrogen source quality/availability	wt and GAT1 (gene from MT2 strain) over-expressing strains were grown for 12, 24, 48 and 72 hours in medium used for fermentation containing 30 g/L glucose, 0.5 g/L MgSO4Â·7H2O, 0.5 g/L KH2PO4, and 5 g/L L-phenylalanine (l-Phe), as a sole nitrogen source.	N/A
			Indirect	RT-PCR - wt vs TF mutant	Positive	YS58	Nitrogen source quality/availability	wt and GAT1 (gene from YS58 strain) over-expressing strains were grown for 12, 24, 48 and 72 hours in medium used for fermentation containing 30 g/L glucose, 0.5 g/L MgSO4Â·7H2O, 0.5 g/L KH2PO4, and 5 g/L L-phenylalanine (l-Phe), as a sole nitrogen source.	N/A
		PubMed Hong J et al., eLife, 2018 Apr 5;7():Hong J et al., eLife, 2018 Apr 5;7():	Indirect	RNA-seq analysis - WT vs TF Mutant	N/A	FY4	Cell cycle/morphology	Early first acidic phase (day 4): Giant colonies grown on GM solid agar (1% yeast extract, 3% glycerol, 2% agar, 30 mM CaCl2) at room temperature; colonies harvested at day 4, corresponding to the early first acidic phase.	-3.223350679
			Indirect	RNA-seq analysis - WT vs TF Mutant	N/A	FY4	Cell cycle/morphology	Middle first acidic phase (day 7): Giant colonies grown on GM solid agar (1% yeast extract, 3% glycerol, 2% agar, 30 mM CaCl2) at room temperature; colonies harvested at day 7, corresponding to the middle first acidic phase.	-2.903040706
			Indirect	RNA-seq analysis - WT vs TF Mutant	N/A	FY4	Unstressed log-phase growth (control)	Grown in synthetic complete (SC) medium at 30°C to a density of 10^7 cells per mL. Transcription factor gene deletion.	-3.671217656
			Indirect	RNA-seq analysis - WT vs TF Mutant	N/A	FY4	Nitrogen source quality/availability	Cells cultured in ammonium-limited chemostats.Strain bearing single GAT1 missense mutations gat1-1 (W321). Transcription factor gene mutation.	-3.223350679
			Indirect	RNA-seq analysis - WT vs TF Mutant	N/A	FY4	Nitrogen source quality/availability	Cells cultured in ammonium-limited chemostats.Strain bearing single GAT1 missense mutations gat1-2 (C331Y). Transcription factor gene mutation.	-2.903040706
			Indirect	RNA-seq analysis - WT vs TF Mutant	N/A	FY4	Nitrogen source quality/availability	Cells cultured in ammonium-limited chemostats.Strain bearing single GAT1 missense mutations gat1-3 (R345G). Transcription factor gene mutation.	-3.671217656