S.cerevisiae - Yeastract

Regulation Reference(s)

The Yeastract team, is actively curating each regulation evidence code, association type and environmental condition.

Transcription Factor	Target ORF/Genes	References	Evidence Code	Evidence Experiment	Association Type	Strain	Environmental Group	Environmental Condition	Log2FC
Gln3p	YFL021W	PubMed Coffman JA et al., Journal of bacteriology, 1997 Jun;179(11):3416-29	Indirect	lacZ - wt vs TF mutant	Positive	TCY1	Nitrogen source quality/availability	Minimal medium + proline	N/A
		PubMed Harbison CT et al., Nature, 2004 Sep 2;431(7004):99-104	Direct	Chip-on-chip	N/A	S288c	Unstressed log-phase growth (control)	SC medium; mid-log phase	N/A
		PubMed Georis I et al., Molecular and cellular biology, 2009 Jul;29(13):3803-15	Indirect	RT-PCR - wt vs TF mutant	Positive	S288c	Nitrogen source quality/availability	proline	N/A
		PubMed Staschke KA et al., The Journal of biological chemistry, 2010 May 28;285(22):16893-911	Indirect	Microarray analysis - WT vs TF Deletion	Positive	WY798	Nitrogen source quality/availability	Grown in synthetic medium (SD) (2% dextrose, 0.5% ammonium sulfate) + amino acids w/o histidine (SC medium). Cells treated for 1 h with 10 mM 3-AT. Transcription factor gene deletion.	0.04264434
			Indirect	Microarray analysis - WT vs TF Deletion	Positive	WY798	Unstressed log-phase growth (control)	Grown in synthetic medium (SD) (2% dextrose, 0.5% ammonium sulfate) + amino acids w/o histidine (SC medium). Transcription factor gene deletion.	0.28273263
		PubMed Georis I et al., The Journal of biological chemistry, 2011 Dec 30;286(52):44897-912	Indirect	RT-PCR - wt vs TF mutant	Positive	S288c	Nitrogen source quality/availability	Methionine sulfoximine	N/A
		PubMed Georis I et al., RNA biology, 2015;12(8):824-37	Indirect	RT-PCR - wt vs TF mutant	Positive	S288c	Nitrogen source quality/availability	wt (TB50) vs gln3DELTA (FV005) mutant, cells grown in YNB medium with proline (Pro) or treated with ammonium and methionine sulfoximine (Am. C Msx); GAT1 expression was assayed using 7 pairs of primers along the open reading frame as shown on the insert	N/A
		PubMed Ponomarova O et al., Cell systems, 2017 Oct 25;5(4):345-357.e6	Indirect	RNA-seq - wt vs TF mutant	Negative	S90	Carbon source quality/availability	wt and DELTAgln3 cells were grown in YPAD medium	N/A
		PubMed Rossi MJ et al., Nature, 2021 Apr;592(7853):309-314	Direct	ChIP-exo	N/A	BY4741	Unstressed log-phase growth (control)	YPD medium, 25.C	N/A
		PubMed Hurieva B et al., Nucleic acids research, 2024 Aug 27;52(15):8763-8777	Direct	ChEC-seq - wt vs TF truncations	N/A	BY4741	Unstressed log-phase growth (control)	SD medium	N/A