PubMed
Cipollina C et al., Microbiology (Reading, England), 2008 Jan;154(Pt 1):337-346Cipollina C et al., Microbiology (Reading, England), 2008 Jan;154(Pt 1):337-346
Indirect
Microarray analysis - WT vs TF Deletion
Positive
SLY34
Unstressed log-phase growth (control)
Grown in rich medium (YPD) at 30 °C to log phase. Transcription factor gene deletion.
0.592608
PubMed
Liang CY et al., Molecular biology of the cell, 2013 Oct;24(20):3251-62Liang CY et al., Molecular biology of the cell, 2013 Oct;24(20):3251-62
Indirect
Microarray analysis - wt vs TF mutant
Positive
S288c
Unstressed log-phase growth (control)
YPD 30.C
N/A
PubMed
Shu WJ et al., Nucleic acids research, 2020 Sep 4;48(15):8360-8373Shu WJ et al., Nucleic acids research, 2020 Sep 4;48(15):8360-8373
Indirect
RNA-seq analysis - WT vs TF Deletion
Positive
BY4741
Carbon source quality/availability
Ethanol medium - Overexpression media lacked leucine to ensure plasmid selection. The cultures grown for 70 h in ethanol medium were diluted twice with a fresh medium to ensure continuous growth.
0.624933058403579
Indirect
RNA-seq analysis - WT vs TF Deletion
Positive
BY4741
Nitrogen source quality/availability
Grown in YPD to OD600 of 0.8 at 30 ºC. Treated with rapamycin for 2h (100 nM). Transcription factor gene deletion.