S.cerevisiae - Yeastract

Regulation Reference(s)

The Yeastract team, is actively curating each regulation evidence code, association type and environmental condition.

Transcription Factor	Target ORF/Genes	References	Evidence Code	Evidence Experiment	Association Type	Strain	Environmental Condition
Leu3p	YGL009C	PubMed Friden P et al., Mol Cell Biol, 1987 Aug;7(8):2708-17	Indirect	Northern blot - WT vs TF mutant	Positive	S288c	basal conditions; rich medium
		PubMed Friden P et al., Mol Cell Biol, 1988 Jul;8(7):2690-7	Indirect	LacZ - WT vs TF mutant	Positive	S288c	SD medium
		PubMed Brisco PR et al., Genetics, 1987 Jan;115(1):91-9	Indirect	Enzyme activity - WT vs TF mutant	Positive	S288c	leucine absence
		PubMed Onda M et al., Gene, 2004;():Onda M et al., Gene, 2004;():	Indirect	Microarray analysis - WT vs TF mutant	Positive	S288c	YPGal + Adenine
		PubMed Harbison CT et al., Nature, 2004 Sep 2;431(7004):99-104	Direct	Chip-on-chip	N/A	S288c	SC medium; mid-log phase
		PubMed Boer VM et al., FEMS Yeast Res, 2005 Jul;5(10):885-97	Indirect	Microarray analysis - WT vs TF mutant	Positive	CEN.PK540-3A	Ethanol-limitation: Defined synthetic medium (NH4)2SO4, 5.0 g/l;KH2PO4,3.0 g/l; MgSO4.7H2O, 0.5 g/l; ethanol, 6.0 g/l; vitamins and trace elements.
		PubMed Tang L et al., BMC Genomics, 2006;():Tang L et al., BMC Genomics, 2006;():	Indirect	Microarray analysis - WT vs TF overexpression	Positive	BY4741	The cells were cultured in uracil dropout medium (YNB-AA (Sigma) 6.7 g/L, 0.77 g/L, Ura DO Supp., and 2% glucose supplemented with G418 at 200 mg/L) with shaking at 30.C. The cells were harvested in log-phase growth (A600 ~ 0.7).
		PubMed Ihrig J et al., Eukaryot Cell, 2010 Mar;9(3):460-71	Indirect	Luciferase - WT vs TF mutant	Positive	S288c	50mM bathophenanthroline (Fe depletion)
		PubMed Reimand J et al., Nucleic Acids Res, 2010 Aug;38(14):4768-77	Indirect	Microarray analysis - WT vs TF mutant	Positive	S288c	YPD medium; mid-log phase
		PubMed Chubukov V et al., Proc Natl Acad Sci U S A, 2012 Mar 27;109(13):5127-32	Indirect	GFP - WT vs TF mutant	Positive	S288c	leucine absence
		PubMed Holland P et al., Nucleic Acids Res, 2019 Jun 4;47(10):4986-5000	Direct	ChIP-exo	N/A	CEN.PK 113-5D	Cells were cultivated in chemostats in anaerobic glucose limited medium with a dilution rate of 0.1 h-1 at 30.C. Samples were collected after steady state was achieved for 48-60 h
			Direct	ChIP-exo	N/A	CEN.PK 113-5D	Cells were cultivated in chemostats in ethanol limited medium with a dilution rate of 0.1 h-1 at 30.C. Samples were collected after steady state was achieved for 48-60 h
			Direct	ChIP-exo	N/A	CEN.PK 113-5D	Cells were cultivated in chemostats in nitrogen limited medium with a dilution rate of 0.1 h-1 at 30.C. Samples were collected after steady state was achieved for 48-60 h
			Direct	ChIP-exo	N/A	CEN.PK 113-5D	Cells were cultivated in chemostats in respiratory glucose limited medium with a dilution rate of 0.1 h-1 at 30.C. Samples were collected after steady state was achieved for 48-60 h
		PubMed Börlin CS et al., FEMS Microbiol Lett, 2020 Jul 1;367(13):	Direct	ChIP-seq	N/A	CEN.PK 113-5D	After 70 h of growth in minimal medium with 2% glucose, at 30.C and 200 rpm, cells were treated with 100 mg/L isoleucine for 2 hours.
			Direct	ChIP-seq	N/A	CEN.PK 113-5D	After 70 h of growth in minimal medium with 2% glucose, at 30.C and 200 rpm, cells were treated with 100 mg/L leucine for 2 hours.
			Direct	ChIP-seq	N/A	CEN.PK 113-5D	After 70 h of growth in minimal medium with 2% glucose, at 30.C and 200 rpm, cells were treated with 100 mg/L valine for 2 hours.
			Direct	ChIP-seq	N/A	CEN.PK 113-5D	After 70 h of growth in minimal medium with 2% glucose, at 30.C and 200 rpm, cells were treated with 20 mg/L leucine for 2 hours.
			Direct	ChIP-seq	N/A	CEN.PK 113-5D	After 70 h of growth in minimal medium with 2% glucose, at 30.C and 200 rpm.
		PubMed Rossi MJ et al., Nature, 2021 Apr;592(7853):309-314	Direct	ChIP-exo	N/A	BY4741	YPD medium, 25.C