The Yeastract team, is actively curating each regulation evidence code, association type and environmental condition.
Transcription
Factor |
Target
ORF/Genes |
References |
Evidence
Code |
Evidence
Experiment |
Association
Type |
Strain |
Environmental
Condition |
Log2FC |
|
Ume6p
|
YGL028C
|
PubMed 
Lardenois A et al., Molecular genetics and genomics : MGG, 2015 Oct;290(5):2031-46Lardenois A et al., Molecular genetics and genomics : MGG, 2015 Oct;290(5):2031-46 |
Indirect |
Microarray analysis - WT vs TF Deletion |
Positive |
SK1 |
Grown in rich media with acetate (YPA) under standard conditions. Transcription factor gene deletion. |
1.090282 |
|
PubMed
Harris A et al., Genetics, 2023 Oct 4;225(2):Harris A et al., Genetics, 2023 Oct 4;225(2): |
Indirect |
RNA-seq analysis - WT vs TF Deletion |
Negative |
SK1 |
Mitotic, 30min: Cells were in mitotic, log-phase growth. They were grown in YPD (1% yeast extract, 2% peptone, 2% dextrose, 22.4 mg/L uracil, 80 mg/L tryptophan) for ~24 h to saturation, then back-diluted into fresh YPD to OD600 = 0.2 and grown ~3 h to log phase (OD600 ≥ 0.5). Once in log phase, β-estradiol (40 nM) was added, followed 30 min later by auxin (200 µM) at t=0min. Sampled at 30 min. |
-1.70895 |
| Indirect |
RNA-seq analysis - WT vs TF Under-expression |
Positive |
SK1 |
Meiotic 2.5h: Cells were transitioning from premeiotic starvation into meiosis. They were grown in YPD for ~24 h to saturation, then inoculated into BYTA (1% yeast extract, 2% bacto tryptone, 1% potassium acetate, 50 mM potassium phthalate) at OD600 = 0.25 and grown 16–18 h at 30 °C to OD600 ≥ 5. Cells were then pelleted, washed with sterile water, and resuspended in SPM (2% KOAc, 0.02% raffinose, amino acid supplements, pH 7) at OD600 = 1.85, shaken at 30 °C and 275 rpm. At 0.5 h in SPM, β-estradiol (5 nM) and auxin (200 µM) were added simultaneously, followed by CuSO4 (50 µM) at 2 h to trigger IME1/4 expression and meiotic entry. Sampled at 2.5 h. |
2.084173 |
| Indirect |
RNA-seq analysis - WT vs TF Under-expression |
Positive |
SK1 |
Meiotic 2h: Cells were transitioning from premeiotic starvation into meiosis. They were grown in YPD for ~24 h to saturation, then inoculated into BYTA (1% yeast extract, 2% bacto tryptone, 1% potassium acetate, 50 mM potassium phthalate) at OD600 = 0.25 and grown 16–18 h at 30 °C to OD600 ≥ 5. Cells were then pelleted, washed with sterile water, and resuspended in SPM (2% KOAc, 0.02% raffinose, amino acid supplements, pH 7) at OD600 = 1.85, shaken at 30 °C and 275 rpm. At 0.5 h in SPM, β-estradiol (5 nM) and auxin (200 µM) were added simultaneously, followed by CuSO4 (50 µM) at 2 h to trigger IME1/4 expression and meiotic entry. Sampled at 2 h. |
0.905836 |
|
